Next Article in Journal
Serum Tumor Markers in Pancreatic Cancer—Recent Discoveries
Previous Article in Journal
Reinventing Diagnostics for Personalized Therapy in Oncology
Article Menu

Export Article

Open AccessArticle
Cancers 2010, 2(2), 1092-1106; doi:10.3390/cancers2021092

Studies on the Non-Protein Thiols of a Human Prostatic Cancer Cell Line: Glutathione Content

CCRF-Mitchell Laboratory, Cambridge Research Laboratories, 181A Huntingdon Rd., Cambridge CB3 ODL, UK
Present address: The Irwin Centre, Scotland Rd., Dry Drayton, Cambridge, CB23 8AR, UK.
Received: 26 April 2010 / Revised: 18 May 2010 / Accepted: 25 May 2010 / Published: 2 June 2010
View Full-Text   |   Download PDF [890 KB, uploaded 2 June 2010]   |  

Abstract

The low molecular weight thiol (-SH) content of a human prostate carcinoma cell line (LNCap), important to the cellular resistance to drugs and irradiation, was investigated using three forms of thiol assay each utilizing different chemistries. The composition of the mixture was examined by derivatization of the thiols with a three-fold excess of the Ellman reagent to give mixed aromatic disulfides. The components were isolated by chromatography on C18 reverse phase silica gel followed by DE52 anion exchange separation, and then analyzed by capillary electrophoresis against prepared standards. The glutathione adduct (GSSE) and an unknown disulfide (RSSE) were the major components isolated on DE52 together with two minor ones. However, from the absorbance at 325 nm, it was found that the GSSE isolated (1.5 ± 0.2 femtomoles/cell) could only account for 28.5 ± 4.3% of the total ASF thiols. It appeared that the bulk of the thiol material had not formed a stable mixed disulfide with Ellman’s reagent, and this was confirmed by 35S labeling of the cells. A large proportion of the 35S labeled components, obtained after reaction of the ASF thiols with the Ellman reagent, did not form mixed aromatic disulfides and could therefore not be identified by this labeling method. View Full-Text
Keywords: human prostate tumor cells; non-protein thiols; Ellman reagent labeling; analysis; glutathione content human prostate tumor cells; non-protein thiols; Ellman reagent labeling; analysis; glutathione content
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Gronow, M. Studies on the Non-Protein Thiols of a Human Prostatic Cancer Cell Line: Glutathione Content. Cancers 2010, 2, 1092-1106.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Cancers EISSN 2072-6694 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top