Non-Invasive Assessment of Lactate Production and Compartmentalization in Renal Cell Carcinomas Using Hyperpolarized 13C Pyruvate MRI
Abstract
:1. Introduction
2. Results
2.1. Orthotopic Tumor Characteristics on 1H MRI and on Histology
2.2. Dynamic HP 13C MRI to Interrogate Pyruvate-to-Lactate Conversion
2.3. Diffusion Weighted HP 13C MRI to Interrogate Lactate Compartmentalization
3. Discussion
4. Materials and Methods
4.1. Cell Lines
4.2. Murine Orthotopic RCC Model
4.3. 1H and HP 13C MRI
4.4. MRI Data Analysis
4.5. LDHA Expression Assay
4.6. H&E and MCT4 Staining, and Imaging Analysis
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sriram, R.; Gordon, J.; Baligand, C.; Ahamed, F.; Delos Santos, J.; Qin, H.; Bok, R.A.; Vigneron, D.B.; Kurhanewicz, J.; Larson, P.E.Z.; et al. Non-Invasive Assessment of Lactate Production and Compartmentalization in Renal Cell Carcinomas Using Hyperpolarized 13C Pyruvate MRI. Cancers 2018, 10, 313. https://doi.org/10.3390/cancers10090313
Sriram R, Gordon J, Baligand C, Ahamed F, Delos Santos J, Qin H, Bok RA, Vigneron DB, Kurhanewicz J, Larson PEZ, et al. Non-Invasive Assessment of Lactate Production and Compartmentalization in Renal Cell Carcinomas Using Hyperpolarized 13C Pyruvate MRI. Cancers. 2018; 10(9):313. https://doi.org/10.3390/cancers10090313
Chicago/Turabian StyleSriram, Renuka, Jeremy Gordon, Celine Baligand, Fayyaz Ahamed, Justin Delos Santos, Hecong Qin, Robert A. Bok, Daniel B. Vigneron, John Kurhanewicz, Peder E. Z. Larson, and et al. 2018. "Non-Invasive Assessment of Lactate Production and Compartmentalization in Renal Cell Carcinomas Using Hyperpolarized 13C Pyruvate MRI" Cancers 10, no. 9: 313. https://doi.org/10.3390/cancers10090313