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Cancers 2018, 10(5), 146; https://doi.org/10.3390/cancers10050146

The Long Non-Coding RNA RP5-1024C24.1 and Its Associated-Gene MPPED2 Are Down-Regulated in Human Thyroid Neoplasias and Act as Tumour Suppressors

1
Institute of Experimental Endocrinology and Oncology (IEOS) “G. Salvatore”, National Research Council (CNR), Via Sergio Pansini 5, 80131 Naples, Italy
2
Department of Molecular Medicine and Medical Biotechnology (DMMBM), University of Naples “Federico II”, Via Sergio Pansini 5, 80131 Naples, Italy
3
Service d’Anatomie et Cytologie Pathologiques, Centre de Biologie Sud, Groupement Hospitalier Lyon Sud, 69495 Pierre Bénite, France
4
CEINGE-Biotecnologie Avanzate, Via Gaetano Salvatore 486, 80145 Naples, Italy
5
Instituto Nacional de Cancer, Laboratorio de Carcinogênese Molecular, Rua Andre Cavalcanti 37, Centro, Rio de Janeiro 20231-050, Brazil
*
Authors to whom correspondence should be addressed.
Received: 3 May 2018 / Revised: 14 May 2018 / Accepted: 15 May 2018 / Published: 18 May 2018
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Abstract

Background: Well-differentiated papillary thyroid carcinoma (PTC) represents the thyroid neoplasia with the highest incidence. Long non-coding RNAs (lncRNAs) have been found deregulated in several human carcinomas, and hence, proposed as potential diagnostic and prognostic markers. Therefore, the aim of our study was to investigate their role in thyroid carcinogenesis. Methods: We analysed the lncRNA expression profile of 12 PTC and four normal thyroid tissues through a lncRNA microarray. Results: We identified 669 up- and 2470 down-regulated lncRNAs with a fold change >2. Among them, we focused on the down-regulated RP5-1024C24.1 located in an antisense position with respect to the MPPED2 gene which codes for a metallophosphoesterase with tumour suppressor activity. Both these genes are down-regulated in benign and malignant thyroid neoplasias. The restoration of RP5-1024C24.1 expression in thyroid carcinoma cell lines reduced cell proliferation and migration by modulating the PTEN/Akt pathway. Inhibition of thyroid carcinoma cell growth and cell migration ability was also achieved by the MPPED2 restoration. Interestingly, RP5-1024C24.1 over-expression is able to increase MPPED2 expression. Conclusions: Taken together, these results demonstrate that RP5-1024C24.1 and MPPED2 might be considered as novel tumour suppressor genes whose loss of expression contributes to thyroid carcinogenesis. View Full-Text
Keywords: long non-coding RNA; MPPED2; thyroid carcinoma; tumour suppressor; carcinogenesis long non-coding RNA; MPPED2; thyroid carcinoma; tumour suppressor; carcinogenesis
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Sepe, R.; Pellecchia, S.; Serra, P.; D’Angelo, D.; Federico, A.; Raia, M.; Cortez Cardoso Penha, R.; Decaussin-Petrucci, M.; Vecchio, L.D.; Fusco, A.; Pallante, P. The Long Non-Coding RNA RP5-1024C24.1 and Its Associated-Gene MPPED2 Are Down-Regulated in Human Thyroid Neoplasias and Act as Tumour Suppressors. Cancers 2018, 10, 146.

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