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Micromachines 2018, 9(1), 7; doi:10.3390/mi9010007

Long-Term Tracking of Free-Swimming Paramecium caudatum in Viscous Media Using a Curved Sample Chamber

1
Department of Mechanical Engineering, Graduate School, Kookmin University, 77 Jeongneung-ro, Seongbuk-gu, Seoul 02707, Korea
2
School of Mechanical Engineering, Kookmin University, 77 Jeongneung-ro, Seongbuk-gu, Seoul 02707, Korea
*
Author to whom correspondence should be addressed.
Received: 31 October 2017 / Revised: 22 December 2017 / Accepted: 26 December 2017 / Published: 28 December 2017
(This article belongs to the Special Issue Locomotion at Small Scales: From Biology to Artificial Systems)
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Abstract

It is technically difficult to acquire large-field images under the complexity and cost restrictions of a diagnostic and instant field research purpose. The goal of the introduced large-field imaging system is to achieve a tolerable resolution for detecting microscale particles or objects in the entire image field without the field-curvature effect, while maintaining a cost-effective procedure and simple design. To use a single commercial lens for imaging a large field, the design attempts to fabricate a curved microfluidic chamber. This imaging technique improves the field curvature and distortion at an acceptable level of particle detection. This study examines Paramecium caudatum microswimmers to track their motion dynamics in different viscous media with imaging techniques. In addition, the study found that the average speed for P. caudatum was 60 µm/s, with a standard deviation of ±12 µm/s from microscopic imaging of the original medium of the sample, which leads to a variation of 20% from the average measurement. In contrast, from large-field imaging, the average speeds of P. caudatum were 63 µm/s and 68 µm/s in the flat and curved chambers, respectively, with the same medium viscosity. Furthermore, the standard deviations that were observed were ±7 µm/s and ±4 µm/s and the variations from the average speed were calculated as 11% and 5.8% for the flat and curved chambers, respectively. The proposed methodology can be applied to measure the locomotion of the microswimmer at small scales with high precision. View Full-Text
Keywords: curved chamber; particle tracking; field curvature; microswimmer; large field of view curved chamber; particle tracking; field curvature; microswimmer; large field of view
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Shourav, M.K.; Kim, J.K. Long-Term Tracking of Free-Swimming Paramecium caudatum in Viscous Media Using a Curved Sample Chamber. Micromachines 2018, 9, 7.

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