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Micromachines 2017, 8(3), 79; doi:10.3390/mi8030079

The Optimization of a Microfluidic CTC Filtering Chip by Simulation

1
Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230031, China
2
Micro&Nano Engineering Laboratory, University of Science and Technology of China, Hefei 230031, China
*
Author to whom correspondence should be addressed.
Academic Editor: Kwang W. Oh
Received: 14 December 2016 / Revised: 18 February 2017 / Accepted: 27 February 2017 / Published: 4 March 2017
(This article belongs to the Special Issue Biomedical Microfluidic Devices)
View Full-Text   |   Download PDF [2502 KB, uploaded 4 March 2017]   |  

Abstract

The detection and separation of circulating tumor cells (CTCs) are crucial in early cancer diagnosis and cancer prognosis. Filtration through a thin film is one of the size and deformability based separation methods, which can isolate rare CTCs from the peripheral blood of cancer patients regardless of their heterogeneity. In this paper, volume of fluid (VOF) multiphase flow models are employed to clarify the cells’ filtering processes. The cells may deform significantly when they enter a channel constriction, which will induce cell membrane stress and damage if the area strain is larger than the critical value. Therefore, the cellular damage criterion characterized by membrane area strain is presented in our model, i.e., the lysis limit of the lipid bilayer is taken as the critical area strain. Under this criterion, we discover that the microfilters with slit-shaped pores do less damage to cells than those with circular pores. The influence of contact angle between the microfilters and blood cells on cellular injury is also discussed. Moreover, the optimal film thickness and flux in our simulations are obtained as 0.5 μm and 0.375 mm/s, respectively. These findings will provide constructive guidance for the improvement of next generation microfilters with higher throughput and less cellular damage. View Full-Text
Keywords: circulating tumor cells (CTCs); circulating tumor cell; microfilter; volume of fluid (VOF); volume of fluid; simulation; area strain; cellular damage circulating tumor cells (CTCs); circulating tumor cell; microfilter; volume of fluid (VOF); volume of fluid; simulation; area strain; cellular damage
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Li, H.; Chen, J.; Du, W.; Xia, Y.; Wang, D.; Zhao, G.; Chu, J. The Optimization of a Microfluidic CTC Filtering Chip by Simulation. Micromachines 2017, 8, 79.

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