A Microfluidic Device for Culturing an Encapsulated Ovarian Follicle
AbstractMicrofluidic chips have been proved effective in mimicking different organs of human body. Simulating human ovarian follicles by microfluidic device will be useful in exploring the mechanism of folliculogenesis and related diseases. In this paper, a microfluidic chip was designed to culture a single human pre-antral follicle. Ovarian follicles were first encapsulated in 3D calcium alginate hydrogel beads and then cultured on chip and in dish under same conditions. The diameters of cultured ovarian follicles were measured, and the same amount of medium was collected from microfluidic device or dish per two days for measuring the estradiol and androgen concentrations. The results confirmed the successful growth of ovarian follicles on chip with their hormonal trends and diameters increase, which were similar to ovarian follicles cultured in dish. It is concluded that this microfluidic chip can be used to culture a single human ovarian follicle, which provides a useful tool to explore the hormonal changes and their interactions during folliculogenesis. View Full-Text
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Aziz, A.U.R.; Fu, M.; Deng, J.; Geng, C.; Luo, Y.; Lin, B.; Yu, X.; Liu, B. A Microfluidic Device for Culturing an Encapsulated Ovarian Follicle. Micromachines 2017, 8, 335.
Aziz AUR, Fu M, Deng J, Geng C, Luo Y, Lin B, Yu X, Liu B. A Microfluidic Device for Culturing an Encapsulated Ovarian Follicle. Micromachines. 2017; 8(11):335.Chicago/Turabian Style
Aziz, Aziz U.R.; Fu, Mengjie; Deng, Jiu; Geng, Chunyang; Luo, Yong; Lin, Bingcheng; Yu, Xiaohui; Liu, Bo. 2017. "A Microfluidic Device for Culturing an Encapsulated Ovarian Follicle." Micromachines 8, no. 11: 335.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.