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Micromachines 2017, 8(1), 1; doi:10.3390/mi8010001

A Microfluidic Chip for Cell Patterning Utilizing Paired Microwells and Protein Patterns

1
Biosensor National Special Laboratory, Key Laboratory of BME of the Ministry of Education, Zhejiang University, Hangzhou 310027, China
2
College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou 310027, China
3
Department of General Surgery, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou 310016, China
4
State Key Laboratory of CAD&CG, Zhejiang University, Hangzhou 310027, China
This paper is an extended version of our paper published in the 2016 International Conference of Microfluidics, Nanofluidics and Lab-on-a-Chip, Dalian, China, 10–12 June 2016.
*
Author to whom correspondence should be addressed.
Academic Editors: Yongxin Song, Junsheng Wang and Dongqing Li
Received: 23 November 2016 / Revised: 14 December 2016 / Accepted: 19 December 2016 / Published: 23 December 2016
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Abstract

Cell patterning has been widely used in research on fundamental cell biology and in applications such as tissue engineering, neuron network formation, cell based biosensor and drug screening. Although various methods have been developed, cell patterning in an enclosed microfluidic device at single cell level remains challenging. This paper describes a microfluidic device with microwells and protein patterns paired together in a single microchannel for an easy cell patterning. Cells captured in the microwells were positioned directly onto the protein patterns within 5 min and the patterning performance was successfully demonstrated using HeLa cells and human gallbladder carcinoma cells (SGC-996). Cells survived for 6 days in the microchannel. Cell attachment, migration, proliferation and cell colony formation were observed. Our device is free of topographic constraint for the patterned cells and no complex chemical modification to the substrate is needed, offering a simple, fast, and easy-to-operate way of patterning cells at single cell level in an enclosed microfluidic channel. View Full-Text
Keywords: microfluidic; microfabrication; lab-on-a-chip; cell patterning; micro contact printing; cell capture; microwell; cell biology microfluidic; microfabrication; lab-on-a-chip; cell patterning; micro contact printing; cell capture; microwell; cell biology
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Tu, C.; Huang, B.; Zhou, J.; Liang, Y.; Tian, J.; Ji, L.; Liang, X.; Ye, X. A Microfluidic Chip for Cell Patterning Utilizing Paired Microwells and Protein Patterns. Micromachines 2017, 8, 1.

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