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Micromachines 2016, 7(6), 101; doi:10.3390/mi7060101

Affinity-Bead-Mediated Enrichment of CD8+ Lymphocytes from Peripheral Blood Progenitor Cell Products Using Acoustophoresis

1
Department of Biomedical Engineering, Lund University, 221 00 Lund, Sweden
2
Department of Laboratory Medicine, Lund Stem Cell Center, Lund University, 221 00 Lund, Sweden
3
Department of Clinical Immunology and Tranfusion medicine, University and Regional Laboratories, 221 85 Lund, Sweden
4
Department of Biomedical Engineering, Dongguk University, 04620 Seoul, Korea
5
Department of Hematology, University Hospital Skåne, 222 41 Lund, Sweden
*
Authors to whom correspondence should be addressed.
Academic Editor: Nam-Trung Nguyen
Received: 14 March 2016 / Revised: 31 May 2016 / Accepted: 2 June 2016 / Published: 9 June 2016
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Abstract

Acoustophoresis is a technique that applies ultrasonic standing wave forces in a microchannel to sort cells depending on their physical properties in relation to the surrounding media. Cell handling and separation for research and clinical applications aims to efficiently separate specific cell populations. Here, we investigated the sorting of CD8 lymphocytes from peripheral blood progenitor cell (PBPC) products by affinity-bead-mediated acoustophoresis. PBPC samples were obtained from healthy donors (n = 4) and patients (n = 18). Mononuclear cells were labeled with anti-CD8-coated magnetic beads and sorted on an acoustophoretic microfluidic device and by standard magnetic cell sorting as a reference method. CD8 lymphocytes were acoustically sorted with a mean purity of 91% ± 8% and a median separation efficiency of 63% (range 15.1%–90.5%) as compared to magnetic sorting (purity 91% ± 14%, recovery 29% (range 5.1%–47.3%)). The viability as well as the proliferation capacity of sorted lymphocytes in the target fraction were unimpaired and, furthermore, hematopoietic progenitor cell assay revealed a preserved clonogenic capacity post-sorting. Bead-mediated acoustophoresis can, therefore, be utilized to efficiently sort less frequent CD8+ lymphocytes from PBPC products in a continuous flow mode while maintaining cell viability and functional capacity of both target and non-target fractions. View Full-Text
Keywords: acoustophoresis; ultrasound; CD8 lymphocytes; magnetic-beads; cell sorting; PBPC; peripheral blood progenitor cells acoustophoresis; ultrasound; CD8 lymphocytes; magnetic-beads; cell sorting; PBPC; peripheral blood progenitor cells
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Urbansky, A.; Lenshof, A.; Dykes, J.; Laurell, T.; Scheding, S. Affinity-Bead-Mediated Enrichment of CD8+ Lymphocytes from Peripheral Blood Progenitor Cell Products Using Acoustophoresis. Micromachines 2016, 7, 101.

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