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Micromachines 2015, 6(4), 409-422; doi:10.3390/mi6040409

Uniform Cell Distribution Achieved by Using Cell Deformation in a Micropillar Array

1
Department of Mechano-Micro Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, G5-27, 4259, Nagatsutacho, Midori-ku, Yokohama, Kanagawa 2268503, Japan
2
Department of Biomolecular Engineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, B-60, 4259, Nagatsutacho, Midori-ku, Yokohama, Kanagawa 2268503, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Joost Lötters
Received: 18 February 2015 / Revised: 10 March 2015 / Accepted: 10 March 2015 / Published: 1 April 2015
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Abstract

The uniform dispersion of cells in a microchamber is important to reproduce results in cellular research. However, achieving this is difficult owing to the laminar flow caused by the small dimensions of such a chamber. In this study, we propose a technique to achieve a uniform distribution of cells using a micropillar array inside a microchamber. The cells deform when they pass through a gap between the micropillars. The deformation causes a repetitive clog-and-release process of cells at the gaps between the micropillars. The micropillar array generates random flow inside the microchamber, resulting in the uniform distribution of the cells via cell accumulation. In the experiment, the distribution of cells in the microchamber with the micropillar array is uniform from end to end, whereas that in the microchamber without the micropillar array is centered. The deviation of the cell distribution from the ideally uniform distribution in the microchamber with the micropillar array is suppressed by 63% compared with that in the microchamber without the micropillar array. The doubling time of the cells passed through the micropillar array did not change relative to that of normal N87 cells. This technique will be helpful for reproducing results in cellular research at the micro scale or for those using microfluidic devices. View Full-Text
Keywords: cell distribution; micro pillar array; micro fluidics cell distribution; micro pillar array; micro fluidics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kaminaga, M.; Ishida, T.; Kadonosono, T.; Kizaka-Kondoh, S.; Omata, T. Uniform Cell Distribution Achieved by Using Cell Deformation in a Micropillar Array. Micromachines 2015, 6, 409-422.

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