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Micromachines 2015, 6(3), 364-374; doi:10.3390/mi6030364

Fast Prototyping of Sensorized Cell Culture Chips and Microfluidic Systems with Ultrashort Laser Pulses

1
Chair of Biophysics, University of Rostock, Rostock 18057, Germany
2
SLV Mecklenburg-Vorpommern GmbH, Rostock 18069, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Nam-Trung Nguyen
Received: 10 February 2015 / Revised: 11 March 2015 / Accepted: 12 March 2015 / Published: 23 March 2015
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Abstract

We developed a confined microfluidic cell culture system with a bottom plate made of a microscopic slide with planar platinum sensors for the measurement of acidification, oxygen consumption, and cell adhesion. The slides were commercial slides with indium tin oxide (ITO) plating or were prepared from platinum sputtering (100 nm) onto a 10-nm titanium adhesion layer. Direct processing of the sensor structures (approximately three minutes per chip) by an ultrashort pulse laser facilitated the production of the prototypes. pH-sensitive areas were produced by the sputtering of 60-nm Si3N4 through a simple mask made from a circuit board material. The system body and polydimethylsiloxane (PDMS) molding forms for the microfluidic structures were manufactured by micromilling using a printed circuit board (PCB) milling machine for circuit boards. The microfluidic structure was finally imprinted in PDMS. Our approach avoided the use of photolithographic techniques and enabled fast and cost-efficient prototyping of the systems. Alternatively, the direct production of metallic, ceramic or polymeric molding tools was tested. The use of ultrashort pulse lasers improved the precision of the structures and avoided any contact of the final structures with toxic chemicals and possible adverse effects for the cell culture in lab-on-a-chip systems. View Full-Text
Keywords: rapid prototyping; micro sensor chip; ITO; oxygen; pH; picosecond laser; cell monitoring system; top-down approach rapid prototyping; micro sensor chip; ITO; oxygen; pH; picosecond laser; cell monitoring system; top-down approach
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Bonk, S.M.; Oldorf, P.; Peters, R.; Baumann, W.; Gimsa, J. Fast Prototyping of Sensorized Cell Culture Chips and Microfluidic Systems with Ultrashort Laser Pulses. Micromachines 2015, 6, 364-374.

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