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Micromachines 2015, 6(11), 1755-1767; doi:10.3390/mi6111454

Using a Microfluidic Gradient Generator to Characterize BG-11 Medium for the Growth of Cyanobacteria Synechococcus elongatus PCC7942

1
Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30013, Taiwan
2
Department of Chemical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan
This paper is an extended version of our paper published in the 10th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE NEMS 2015), Xi’an, China, 7–11 April 2015.
*
Authors to whom correspondence should be addressed.
Academic Editors: Wen Jung Li and Ting-Hsuan Chen
Received: 2 September 2015 / Revised: 31 October 2015 / Accepted: 5 November 2015 / Published: 13 November 2015
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Abstract

The photosynthetic cyanobacterium Synechococcus elongatus PCC7942 has recently gained great attention for its ability to directly convert CO2 into renewable chemicals upon genetic engineering. Thus, it is of great interest to increase the growth speed and lower the medium requirement for cultivating this cyanobacterium. The cultivation medium of Synechococcus elongatus PCC7942 has been developed, which consists of many inorganic and metal ingredients with a specific composition, known as the BG-11 medium. In this work, we analyzed the concentration effect of each ingredient and identified the absolutely essential components in BG-11 medium for cyanobacteria growth using the concentration gradient generator chip (CGGC) fabricated by MEMS technology. As shown by our results, removal of the individual component sodium nitrate, potassium phosphate, or magnesium sulfate from the BG-11 medium led to severe growth inhibition of Synechococcus elongatus PCC7942. Contrary to our expectation, increasing concentration of the crucial ingredients showed either insignificant or negative impact on cell growth. Overall, standard growth could be achieved without supplementation of ethylenediaminetetraacetic acid (EDTA) disodium, sodium carbonate, or sodium citrate to the culture medium. Further improvement of the CGGC-based microfluidic system based on this preliminary study may broaden its application range to analyze more complicated correlations. View Full-Text
Keywords: cyanobacterium Synechococcus elongatus PCC7942; BG-11 medium characterization; growth optimization; microfluidic concentration gradient generator chip cyanobacterium Synechococcus elongatus PCC7942; BG-11 medium characterization; growth optimization; microfluidic concentration gradient generator chip
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Yang, C.-C.; Wen, R.C.; Shen, C.R.; Yao, D.-J. Using a Microfluidic Gradient Generator to Characterize BG-11 Medium for the Growth of Cyanobacteria Synechococcus elongatus PCC7942. Micromachines 2015, 6, 1755-1767.

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