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Micromachines 2012, 3(3), 574-581; doi:10.3390/mi3030574
Communication

Very High Throughput Electrical Cell Lysis and Extraction of Intracellular Compounds Using 3D Carbon Electrodes in Lab-on-a-Chip Devices

1,†
,
1,†,* , 2
,
2
 and
1
1 Microsystems Laboratory LMIS4, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 17, CH-1015 Lausanne, Switzerland 2 Swiss Institute for Experimental Cancer Research, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 19, Lausanne, Vaud 1015, Switzerland These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 25 June 2012 / Revised: 30 July 2012 / Accepted: 3 August 2012 / Published: 16 August 2012
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Abstract

Here we present an electrical lysis throughput of 600 microliters per minute at high cell density (108 yeast cells per ml) with 90% efficiency, thus improving the current common throughput of one microliter per minute. We also demonstrate the extraction of intracellular luciferase from mammalian cells with efficiency comparable to off-chip bulk chemical lysis. The goal of this work is to develop a sample preparation module that can act as a stand-alone device or be integrated to other functions already demonstrated in miniaturized devices, including sorting and analysis, towards a true lab-on-a-chip.
Keywords: microfluidics; cell lysis; carbon electrodes microfluidics; cell lysis; carbon electrodes
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Mernier, G.; Martinez-Duarte, R.; Lehal, R.; Radtke, F.; Renaud, P. Very High Throughput Electrical Cell Lysis and Extraction of Intracellular Compounds Using 3D Carbon Electrodes in Lab-on-a-Chip Devices. Micromachines 2012, 3, 574-581.

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