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Micromachines 2012, 3(3), 574-581; doi:10.3390/mi3030574
Communication

Very High Throughput Electrical Cell Lysis and Extraction of Intracellular Compounds Using 3D Carbon Electrodes in Lab-on-a-Chip Devices

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Received: 25 June 2012 / Revised: 30 July 2012 / Accepted: 3 August 2012 / Published: 16 August 2012
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Abstract

Here we present an electrical lysis throughput of 600 microliters per minute at high cell density (108 yeast cells per ml) with 90% efficiency, thus improving the current common throughput of one microliter per minute. We also demonstrate the extraction of intracellular luciferase from mammalian cells with efficiency comparable to off-chip bulk chemical lysis. The goal of this work is to develop a sample preparation module that can act as a stand-alone device or be integrated to other functions already demonstrated in miniaturized devices, including sorting and analysis, towards a true lab-on-a-chip.
Keywords: microfluidics; cell lysis; carbon electrodes microfluidics; cell lysis; carbon electrodes
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mernier, G.; Martinez-Duarte, R.; Lehal, R.; Radtke, F.; Renaud, P. Very High Throughput Electrical Cell Lysis and Extraction of Intracellular Compounds Using 3D Carbon Electrodes in Lab-on-a-Chip Devices. Micromachines 2012, 3, 574-581.

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