Next Article in Journal
Awareness and Prevalence of Mycotoxin Contamination in Selected Nigerian Fermented Foods
Previous Article in Journal
Efficacy of Bee Venom Acupuncture for Chronic Low Back Pain: A Randomized, Double-Blinded, Sham-Controlled Trial
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Toxins 2017, 9(11), 360; https://doi.org/10.3390/toxins9110360

Effects of Lonomia obliqua Venom on Vascular Smooth Muscle Cells: Contribution of NADPH Oxidase-Derived Reactive Oxygen Species

1
Laboratório de Biologia RedOx, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro CEP 21941-902, Brazil
2
Laboratório de Farmacologia Celular e Molecular, Departamento de Biologia Celular, Universidade do Estado do Rio de Janeiro, Rio de Janeiro CEP 20550-030, Brazil
3
Laboratório de Bioquímica Farmacológica, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (CPE/HCPA/UFRGS), Porto Alegre CEP 90035-903, Brazil
4
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre CEP 951501-970, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Angel A. Yanagihara
Received: 8 October 2017 / Revised: 25 October 2017 / Accepted: 3 November 2017 / Published: 7 November 2017
(This article belongs to the Section Animal Venoms)
View Full-Text   |   Download PDF [2303 KB, uploaded 7 November 2017]   |  

Abstract

Envenomation caused by human contact with the caterpillar Lonomia is characterized by deleterious effects on coagulation and patency of blood vessels. The cellular effects induced by Lonomia obliqua venom highlights its capacity to activate endothelial cells, leading to a proinflammatory phenotype. Having more knowledge about the mechanisms involved in envenomation may contribute to better treatment. We aimed to evaluate the effects of Lonomia obliqua caterpillar bristle extract (LOCBE) on vascular smooth muscle cells (VSMC). We observed that LOCBE induced VSMC migration, which was preceded by alterations in actin cytoskeleton dynamics and Focal Adhesion Kinase activation. LOCBE also induced Extracellular Signal-Regulated Kinase (ERK) phosphorylation in VSMC, and the inhibition of this pathway impaired cell proliferation. Stimulation of VSMC with LOCBE triggered reactive oxygen species (ROS) production through the activation of NADPH oxidase. The rapid increase in these ROS further induced mitochondrial ROS production, however only NADPH oxidase-derived ROS were involved in ERK activation in VSMC. We that demonstrated the chemotactic and proliferative effects of LOCBE on VSMC were dependent on ROS production, mainly through NADPH oxidase. Together, the data show that Lonomia obliqua venom can interact with and activate VSMC. These effects rely on ROS production, suggesting new potential targets for treatment against vascular damage during envenomation. View Full-Text
Keywords: Lonomia obliqua venom; vascular smooth muscle cells; reactive oxygen species Lonomia obliqua venom; vascular smooth muscle cells; reactive oxygen species
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Moraes, J.A.; Rodrigues, G.; Nascimento-Silva, V.; Renovato-Martins, M.; Berger, M.; Guimarães, J.A.; Barja-Fidalgo, C. Effects of Lonomia obliqua Venom on Vascular Smooth Muscle Cells: Contribution of NADPH Oxidase-Derived Reactive Oxygen Species. Toxins 2017, 9, 360.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top