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Toxins 2017, 9(11), 360; doi:10.3390/toxins9110360

Effects of Lonomia obliqua Venom on Vascular Smooth Muscle Cells: Contribution of NADPH Oxidase-Derived Reactive Oxygen Species

Laboratório de Biologia RedOx, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro CEP 21941-902, Brazil
Laboratório de Farmacologia Celular e Molecular, Departamento de Biologia Celular, Universidade do Estado do Rio de Janeiro, Rio de Janeiro CEP 20550-030, Brazil
Laboratório de Bioquímica Farmacológica, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (CPE/HCPA/UFRGS), Porto Alegre CEP 90035-903, Brazil
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre CEP 951501-970, Brazil
Author to whom correspondence should be addressed.
Academic Editor: Angel A. Yanagihara
Received: 8 October 2017 / Revised: 25 October 2017 / Accepted: 3 November 2017 / Published: 7 November 2017
(This article belongs to the Section Animal Venoms)
View Full-Text   |   Download PDF [2303 KB, uploaded 7 November 2017]   |  


Envenomation caused by human contact with the caterpillar Lonomia is characterized by deleterious effects on coagulation and patency of blood vessels. The cellular effects induced by Lonomia obliqua venom highlights its capacity to activate endothelial cells, leading to a proinflammatory phenotype. Having more knowledge about the mechanisms involved in envenomation may contribute to better treatment. We aimed to evaluate the effects of Lonomia obliqua caterpillar bristle extract (LOCBE) on vascular smooth muscle cells (VSMC). We observed that LOCBE induced VSMC migration, which was preceded by alterations in actin cytoskeleton dynamics and Focal Adhesion Kinase activation. LOCBE also induced Extracellular Signal-Regulated Kinase (ERK) phosphorylation in VSMC, and the inhibition of this pathway impaired cell proliferation. Stimulation of VSMC with LOCBE triggered reactive oxygen species (ROS) production through the activation of NADPH oxidase. The rapid increase in these ROS further induced mitochondrial ROS production, however only NADPH oxidase-derived ROS were involved in ERK activation in VSMC. We that demonstrated the chemotactic and proliferative effects of LOCBE on VSMC were dependent on ROS production, mainly through NADPH oxidase. Together, the data show that Lonomia obliqua venom can interact with and activate VSMC. These effects rely on ROS production, suggesting new potential targets for treatment against vascular damage during envenomation. View Full-Text
Keywords: Lonomia obliqua venom; vascular smooth muscle cells; reactive oxygen species Lonomia obliqua venom; vascular smooth muscle cells; reactive oxygen species

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Moraes, J.A.; Rodrigues, G.; Nascimento-Silva, V.; Renovato-Martins, M.; Berger, M.; Guimarães, J.A.; Barja-Fidalgo, C. Effects of Lonomia obliqua Venom on Vascular Smooth Muscle Cells: Contribution of NADPH Oxidase-Derived Reactive Oxygen Species. Toxins 2017, 9, 360.

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