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Toxins 2016, 8(7), 198; doi:10.3390/toxins8070198

Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Survive in Kupffer Cells and Exhibit High Virulence in Mice

1
Department of Plastic, Reconstructive and Aesthetic Surgery, Faculty of Medicine, Fukuoka University, Fukuoka 814-0180, Japan
2
Department of Pharmacy, Fukuoka University Chikushi Hospital, Chikusino 818-8502, Japan
3
Department of Medical Oncology, Hematology, and Infectious Diseases, Faculty of Medicine, Fukuoka University, Fukuoka 814-0180, Japan
4
Central Laboratory for Pathology and Morphology, Department of Pathology, Faculty of Medicine, Fukuoka University, Fukuoka 814-0180, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Vernon L. Tesh
Received: 28 March 2016 / Revised: 15 June 2016 / Accepted: 24 June 2016 / Published: 30 June 2016
(This article belongs to the Collection Staphylococcus aureus Toxins)
View Full-Text   |   Download PDF [9212 KB, uploaded 30 June 2016]   |  

Abstract

Although Staphylococcus aureus is part of the normal body flora, heavy usage of antibiotics has resulted in the emergence of methicillin-resistant strains (MRSA). MRSA can form biofilms and cause indwelling foreign body infections, bacteremia, soft tissue infections, endocarditis, and osteomyelitis. Using an in vitro assay, we screened 173 clinical blood isolates of MRSA and selected 20 high-biofilm formers (H-BF) and low-biofilm formers (L-BF). These were intravenously administered to mice and the general condition of mice, the distribution of bacteria, and biofilm in the liver, lung, spleen, and kidney were investigated. MRSA count was the highest in the liver, especially within Kupffer cells, which were positive for acid polysaccharides that are associated with intracellular biofilm. After 24 h, the general condition of the mice worsened significantly in the H-BF group. In the liver, bacterial deposition and aggregation and the biofilm-forming spot number were all significantly greater for H-BF group than for L-BF. CFU analysis revealed that bacteria in the H-BF group survived for long periods in the liver. These results indicate that the biofilm-forming ability of MRSA is a crucial factor for intracellular persistence, which could lead to chronic infections. View Full-Text
Keywords: MRSA; virulence; biofilm; intracellular persistence; Kupffer cells; mice MRSA; virulence; biofilm; intracellular persistence; Kupffer cells; mice
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Oyama, T.; Miyazaki, M.; Yoshimura, M.; Takata, T.; Ohjimi, H.; Jimi, S. Biofilm-Forming Methicillin-Resistant Staphylococcus aureus Survive in Kupffer Cells and Exhibit High Virulence in Mice. Toxins 2016, 8, 198.

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