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Toxins 2016, 8(4), 92; doi:10.3390/toxins8040092

Rapid Detection of Escherichia coli O157 and Shiga Toxins by Lateral Flow Immunoassays

Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, 115 Henning Building, University Park, PA 16802, USA
Present address: Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, China.
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Author to whom correspondence should be addressed.
Academic Editor: Vernon Tesh
Received: 29 January 2016 / Revised: 18 March 2016 / Accepted: 22 March 2016 / Published: 25 March 2016
(This article belongs to the Collection Rapid Detection of Bacterial Toxins)
View Full-Text   |   Download PDF [1351 KB, uploaded 25 March 2016]   |  

Abstract

Shiga toxin-producing Escherichia coli O157:H7 (STEC) cause food-borne illness that may be fatal. STEC strains enumerate two types of potent Shiga toxins (Stx1 and Stx2) that are responsible for causing diseases. It is important to detect the E. coli O157 and Shiga toxins in food to prevent outbreak of diseases. We describe the development of two multi-analyte antibody-based lateral flow immunoassays (LFIA); one for the detection of Stx1 and Stx2 and one for the detection of E. coli O157 that may be used simultaneously to detect pathogenic E. coli O157:H7. The LFIA strips were developed by conjugating nano colloidal gold particles with monoclonal antibodies against Stx1 and Stx2 and anti-lipid A antibodies to capture Shiga toxins and O157 antigen, respectively. Our results indicate that the LFIA for Stx is highly specific and detected Stx1 and Stx2 within three hours of induction of STEC with ciprofloxacin at 37 °C. The limit of detection for E. coli O157 LFIA was found to be 105 CFU/mL in ground beef spiked with the pathogen. The LFIAs are rapid, accurate and easy to use and do not require sophisticated equipment or trained personnel. Following the assay, colored bands on the membrane develop for end-point detection. The LFIAs may be used for screening STEC in food and the environment. View Full-Text
Keywords: lateral flow immunoassay (LFIA); colloidal gold; E. coli O157; Shiga toxin; Stx; STEC; food safety lateral flow immunoassay (LFIA); colloidal gold; E. coli O157; Shiga toxin; Stx; STEC; food safety
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wang, J.; Katani, R.; Li, L.; Hegde, N.; Roberts, E.L.; Kapur, V.; DebRoy, C. Rapid Detection of Escherichia coli O157 and Shiga Toxins by Lateral Flow Immunoassays. Toxins 2016, 8, 92.

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