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Toxins 2016, 8(1), 13; doi:10.3390/toxins8010013

Rapid Microfluidic Assay for the Detection of Botulinum Neurotoxin in Animal Sera

1
Produce Safety & Microbiology Research Unit, United States Department of Agriculture, Agricultural Research Service, 800 Buchanan St, Albany, CA 94710, USA
2
Foodborne Toxin Detection and Prevention Unit, United States Department of Agriculture, Agricultural Research Service, 800 Buchanan St, Albany, CA 94710, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Daniel Gillet
Received: 9 December 2015 / Revised: 23 December 2015 / Accepted: 25 December 2015 / Published: 4 January 2016
(This article belongs to the Collection Rapid Detection of Bacterial Toxins)
View Full-Text   |   Download PDF [2100 KB, uploaded 4 January 2016]   |  

Abstract

Potent Botulinum neurotoxins (BoNTs) represent a threat to public health and safety. Botulism is a disease caused by BoNT intoxication that results in muscle paralysis that can be fatal. Sensitive assays capable of detecting BoNTs from different substrates and settings are essential to limit foodborne contamination and morbidity. In this report, we describe a rapid 96-well microfluidic double sandwich immunoassay for the sensitive detection of BoNT-A from animal sera. This BoNT microfluidic assay requires only 5 μL of serum, provides results in 75 min using a standard fluorescence microplate reader and generates minimal hazardous waste. The assay has a <30 pg·mL−1 limit of detection (LOD) of BoNT-A from spiked human serum. This sensitive microfluidic BoNT-A assay offers a fast and simplified workflow suitable for the detection of BoNT-A from serum samples of limited volume in most laboratory settings. View Full-Text
Keywords: toxin; botulinum neurotoxin (BoNT); botulism; microfluidic; immunoassay; rapid detection; animal serum; diagnostic toxin; botulinum neurotoxin (BoNT); botulism; microfluidic; immunoassay; rapid detection; animal serum; diagnostic
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Babrak, L.; Lin, A.; Stanker, L.H.; McGarvey, J.; Hnasko, R. Rapid Microfluidic Assay for the Detection of Botulinum Neurotoxin in Animal Sera. Toxins 2016, 8, 13.

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