Detection of Shiga Toxins by Lateral Flow Assay
AbstractShiga toxin-producing Escherichia coli (STEC) produce shiga toxins (Stxs) that can cause human disease and death. The contamination of food products with STEC represents a food safety problem that necessitates rapid and effective detection strategies to mitigate risk. In this manuscript, we report the development of a colorimetric lateral flow assay (LFA) for the rapid detection of Stxs in <10 min using a pair of monoclonal antibodies that bind epitopes common to Stx1 and six Stx2 variants. This LFA provides a rapid and sensitive test for the detection of Stxs directly from STEC culture supernatants or at risk food samples with a 0.1 ng/mL limit of detection (LOD) for Stx2a. This Stx LFA is applicable for use in the rapid evaluation of Stx production from cultured E. coli strains or as a tool to augment current methods as part of food safety testing. View Full-Text
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Ching, K.H.; He, X.; Stanker, L.H.; Lin, A.V.; McGarvey, J.A.; Hnasko, R. Detection of Shiga Toxins by Lateral Flow Assay. Toxins 2015, 7, 1163-1173.
Ching KH, He X, Stanker LH, Lin AV, McGarvey JA, Hnasko R. Detection of Shiga Toxins by Lateral Flow Assay. Toxins. 2015; 7(4):1163-1173.Chicago/Turabian Style
Ching, Kathryn H.; He, Xiaohua; Stanker, Larry H.; Lin, Alice V.; McGarvey, Jeffery A.; Hnasko, Robert. 2015. "Detection of Shiga Toxins by Lateral Flow Assay." Toxins 7, no. 4: 1163-1173.