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Toxins 2015, 7(4), 1163-1173; doi:10.3390/toxins7041163

Detection of Shiga Toxins by Lateral Flow Assay

1
Produce Safety & Microbiology Research Unit, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St, Albany, CA 94710, USA
2
Foodborne Toxin Detection and Prevention Research Unit, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan St, Albany, CA 94710, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Gerald B. Koudelka
Received: 4 March 2015 / Revised: 26 March 2015 / Accepted: 30 March 2015 / Published: 3 April 2015
(This article belongs to the Collection Shiga Toxins)
View Full-Text   |   Download PDF [697 KB, uploaded 3 April 2015]   |  

Abstract

Shiga toxin-producing Escherichia coli (STEC) produce shiga toxins (Stxs) that can cause human disease and death. The contamination of food products with STEC represents a food safety problem that necessitates rapid and effective detection strategies to mitigate risk. In this manuscript, we report the development of a colorimetric lateral flow assay (LFA) for the rapid detection of Stxs in <10 min using a pair of monoclonal antibodies that bind epitopes common to Stx1 and six Stx2 variants. This LFA provides a rapid and sensitive test for the detection of Stxs directly from STEC culture supernatants or at risk food samples with a 0.1 ng/mL limit of detection (LOD) for Stx2a. This Stx LFA is applicable for use in the rapid evaluation of Stx production from cultured E. coli strains or as a tool to augment current methods as part of food safety testing. View Full-Text
Keywords: shiga toxin; Stx; toxin; STEC; E. coli O157; immunoassay; lateral flow assay; food safety shiga toxin; Stx; toxin; STEC; E. coli O157; immunoassay; lateral flow assay; food safety
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Ching, K.H.; He, X.; Stanker, L.H.; Lin, A.V.; McGarvey, J.A.; Hnasko, R. Detection of Shiga Toxins by Lateral Flow Assay. Toxins 2015, 7, 1163-1173.

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