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Toxins 2015, 7(3), 705-718; doi:10.3390/toxins7030705

Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study

Area of Toxicology, Department of Public Health, Faculty of Pharmacy, University of Valencia, Burjassot 46100, Spain
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editor: Sven Dänicke
Received: 16 December 2014 / Revised: 12 February 2015 / Accepted: 16 February 2015 / Published: 25 February 2015
(This article belongs to the Collection Fusarium Toxins – Relevance for Human and Animal Health)
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Abstract

A duplicate diet study was designed to explore the occurrence of 15 Fusarium mycotoxins in the 24 h-diet consumed by one volunteer as well as the levels of mycotoxins in his 24 h-collected urine. The employed methodology involved solvent extraction at high ionic strength followed by dispersive solid phase extraction and gas chromatography determination coupled to mass spectrometry in tandem. Satisfactory results in method performance were achieved. The method’s accuracy was in a range of 68%–108%, with intra-day relative standard deviation and inter-day relative standard deviation lower than 12% and 15%, respectively. The limits of quantitation ranged from 0.1 to 8 µg/Kg. The matrix effect was evaluated and matrix-matched calibrations were used for quantitation. Only deoxynivalenol (DON) was quantified in both food and urine samples. A total DON daily intake amounted to 49.2 ± 5.6 µg whereas DON daily excretion of 35.2 ± 4.3 µg was determined. DON daily intake represented 68.3% of the established DON provisional maximum tolerable daily intake (PMTDI). Valuable preliminary information was obtained as regards DON excretion and needs to be confirmed in large-scale monitoring studies. View Full-Text
Keywords: deoxynivalenol; duplicate diet study; GC-MS/MS; urine; risk characterization deoxynivalenol; duplicate diet study; GC-MS/MS; urine; risk characterization
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Rodríguez-Carrasco, Y.; Mañes, J.; Berrada, H.; Font, G. Preliminary Estimation of Deoxynivalenol Excretion through a 24 h Pilot Study. Toxins 2015, 7, 705-718.

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