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Addendum published on 11 June 2015, see Toxins 2015, 7(6), 2134.

Open AccessArticle
Toxins 2015, 7(2), 238-254; doi:10.3390/toxins7020238

Rapid Multiple Immunoenzyme Assay of Mycotoxins

A.N. Bach Institute of Biochemistry of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia
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Author to whom correspondence should be addressed.
Academic Editor: Laura Anfossi
Received: 12 November 2014 / Revised: 19 December 2014 / Accepted: 16 January 2015 / Published: 27 January 2015
(This article belongs to the Section Mycotoxins)
View Full-Text   |   Download PDF [1066 KB, uploaded 27 January 2015]   |  

Abstract

Mycotoxins are low molecular weight fungal metabolites that pose a threat as toxic contaminants of food products, thereby necessitating their effective monitoring and control. Microplate ELISA can be used for this purpose, but this method is characteristically time consuming, with a duration extending to several hours. This report proposes a variant of the ELISA method for the detection and quantification of three mycotoxins, ochratoxin A, aflatoxin B1 and zearalenone, in the kinetic regime. The main requirement for the proposed kinetic protocol was to provide a rapid method that combined sensitivity and accuracy. The use of biotin with an extended spacer together with a streptavidin–polyperoxidase conjugate provided high signal levels, despite these interactions occurring under non-equilibrium conditions. Duration of the individual mycotoxin assays was 20 min, whereas the analysis of all three mycotoxins in parallel reached a maximum duration of 25 min. Recovery of at least 95% mycotoxins in water-organic extracts was shown. The developed assays were successfully validated using poultry processing products and corn samples spiked with known quantities of mycotoxins. The detection limits for aflatoxin B1, ochratoxin A and zearalenone in these substances were 0.24, 1.2 and 3 ng/g, respectively. View Full-Text
Keywords: mycotoxins; zearalenone; aflatoxin B1; ochratoxin A; ELISA; kinetic immunoassay; biotin-streptavidin reaction; methanol extracts mycotoxins; zearalenone; aflatoxin B1; ochratoxin A; ELISA; kinetic immunoassay; biotin-streptavidin reaction; methanol extracts
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Urusov, A.E.; Zherdev, A.V.; Petrakova, A.V.; Sadykhov, E.G.; Koroleva, O.V.; Dzantiev, B.B. Rapid Multiple Immunoenzyme Assay of Mycotoxins. Toxins 2015, 7, 238-254.

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