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Toxins 2015, 7(10), 4381-4389; doi:10.3390/toxins7104381

Quantitative Detection of Shiga Toxins Directly from Stool Specimens of Patients Associated with an Outbreak of Enterohemorrhagic Escherichia coli in Japan—Quantitative Shiga toxin detection from stool during EHEC outbreak

1
Division of Food Hygiene, Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-11, Inada-cho, Obihiro 080-8555, Hokkaido, Japan
2
Toyama Institute of Health, Imizu, Toyama 939-0363, Japan
3
Translational Health Science and Technology Institute, Faridabad, Haryana 121001, India
*
Author to whom correspondence should be addressed.
Academic Editor: Xiaohua He
Received: 15 July 2015 / Revised: 21 October 2015 / Accepted: 22 October 2015 / Published: 27 October 2015
(This article belongs to the Collection Rapid Detection of Bacterial Toxins)
View Full-Text   |   Download PDF [469 KB, uploaded 27 October 2015]   |  

Abstract

Detection of Shiga toxins (Stx) is important for accurate diagnosis of Enterohemorrhagic Escherichia coli infection. In this study, we quantitatively analyzed Stx protein in nine patients’ stool during an outbreak that occurred in Japan. Highly sensitive immunoassay (bead enzyme-linked immunosorbent assay (bead-ELISA)) revealed that the concentrations of toxins in stool of patients ranged from 0.71 to 10.44 ng/mL for Stx1 and 2.75 to 51.61 ng/mL for Stx2. To our knowledge, this is the first report that reveals the range of Stx protein concentrations in human stools. View Full-Text
Keywords: Shiga toxin; Enterohemorrhagic Escherichia coli outbreak; rapid detection; bead enzyme-linked immunosorbent assay Shiga toxin; Enterohemorrhagic Escherichia coli outbreak; rapid detection; bead enzyme-linked immunosorbent assay
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Yamasaki, E.; Watahiki, M.; Isobe, J.; Sata, T.; Nair, G.B.; Kurazono, H. Quantitative Detection of Shiga Toxins Directly from Stool Specimens of Patients Associated with an Outbreak of Enterohemorrhagic Escherichia coli in Japan—Quantitative Shiga toxin detection from stool during EHEC outbreak. Toxins 2015, 7, 4381-4389.

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