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Toxins 2015, 7(10), 3989-4005; doi:10.3390/toxins7103989

Cytotoxic Activity and Antiproliferative Effects of Crude Skin Secretion from Physalaemus nattereri (Anura: Leptodactylidae) on in vitro Melanoma Cells

1
Laboratory of Toxinology, Department of Physiological Sciences/IB, University of Brasília, Brasília/DF, CEP 70.910-900, Brazil
2
Laboratory of Biochemistry and Protein Chemistry, Department of Cell Biology/IB, University of Brasília, Brasília/DF, CEP 70.910-900, Brazil
3
Department of Genetics and Morphology/IB, University of Brasília, Brasília/DF, CEP 70.910-900, Brazil
4
Faculty of Ceilândia, University of Brasília, Ceilândia/DF, CEP 72.220-140, Brazil
These authors contributed equally to this study.
*
Author to whom correspondence should be addressed.
Academic Editor: Richard Lewis
Received: 4 June 2015 / Revised: 24 August 2015 / Accepted: 27 August 2015 / Published: 8 October 2015
(This article belongs to the Section Animal Venoms)
View Full-Text   |   Download PDF [1814 KB, uploaded 8 October 2015]   |  

Abstract

Anuran secretions are rich sources of bioactive molecules, including antimicrobial and antitumoral compounds. The aims of this study were to investigate the therapeutic potential of Physalaemus nattereri skin secretion against skin cancer cells, and to assess its cytotoxic action mechanisms on the murine melanoma cell line B16F10. Our results demonstrated that the crude secretion reduced the viability of B16F10 cells, causing changes in cell morphology (e.g., round shape and structure shrinkage), reduction in mitochondrial membrane potential, increase in phosphatidylserine exposure, and cell cycle arrest in S-phase. Together, these changes suggest that tumor cells die by apoptosis. This skin secretion was also subjected to chromatographic fractioning using RP-HPLC, and eluted fractions were assayed for antiproliferative and antibacterial activities. Three active fractions showed molecular mass components in a range compatible with peptides. Although the specific mechanisms causing the reduced cell viability and cytotoxicity after the treatment with crude secretion are still unknown, it may be considered that molecules, such as the peptides found in the secretion, are effective against B16F10 tumor cells. Considering the growing need for new anticancer drugs, data presented in this study strongly reinforce the validity of P. nattereri crude secretion as a rich source of new anticancer molecules. View Full-Text
Keywords: anurans; antitumoral activity; Physalaemus nattereri; skin secretion; melanoma; cytotoxicity anurans; antitumoral activity; Physalaemus nattereri; skin secretion; melanoma; cytotoxicity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Carvalho, A.C.; Márquez, C.A.P.; Azevedo, R.B.; Joanitti, G.A.; Júnior, O.R.P.; Fontes, W.; Castro, M.S. Cytotoxic Activity and Antiproliferative Effects of Crude Skin Secretion from Physalaemus nattereri (Anura: Leptodactylidae) on in vitro Melanoma Cells. Toxins 2015, 7, 3989-4005.

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