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Toxins 2014, 6(9), 2694-2707; doi:10.3390/toxins6092694

Inheritance Patterns, Dominance and Cross-Resistance of Cry1Ab- and Cry1Ac-Selected Ostrinia furnacalis (Guenée)

1
The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2
Newcastle Institute for Research on Sustainability, School of Biology, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK
3
Agronomy College, Sichuan Agricultural University, Chengdu 611130, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 30 April 2014 / Revised: 13 August 2014 / Accepted: 18 August 2014 / Published: 11 September 2014
(This article belongs to the Special Issue Bacillus thuringiensis Toxins)
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Abstract

Two colonies of Asian corn borer, Ostrinia furnacalis (Guenée), artificially selected from a Bt-susceptible colony (ACB-BtS) for resistance to Cry1Ab (ACB-AbR) and Cry1Ac (ACB-AcR) toxins, were used to analyze inheritance patterns of resistance to Cry1 toxins. ACB-AbR and ACB-AcR evolved significant levels of resistance, with resistance ratios (RR) of 39-fold and 78.8-fold to Cry1Ab and Cry1Ac, respectively. The susceptibility of ACB-AbR larvae to Cry1Ac and Cry1F toxins, which had not previously been exposed, were significantly reduced, being >113-fold and 48-fold, respectively. Similarly, susceptibility of ACB-AcR larvae to Cry1Ab and Cry1F were also significantly reduced (RR > nine-fold, RR > 18-fold, respectively), indicating cross-resistance among Cry1Ab, Cry1Ac, and Cry1F toxins. However, ACB-AbR and ACB-AcR larvae were equally susceptible to Cry1Ie as were ACB-BtS larvae, indicating no cross-resistance between Cry1Ie and Cry1Ab or Cry1Ac toxins; this may provide considerable benefits in preventing or delaying the evolution of resistance in ACB to Cry1Ab and Cry1Ac toxins. Backcrossing studies indicated that resistance to Cry1Ab toxin was polygenic in ACB-AbR, but monogenic in ACB-AcR, whilst resistance to Cry1Ac toxin was primarily monogenic in both ACB-AbR and ACB-AcR, but polygenic as resistance increased. View Full-Text
Keywords: Ostrinia furnacalis; Cry1 toxins; cross-resistance; resistance inheritance Ostrinia furnacalis; Cry1 toxins; cross-resistance; resistance inheritance
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MDPI and ACS Style

Zhang, T.; He, M.; Gatehouse, A.M.R.; Wang, Z.; Edwards, M.G.; Li, Q.; He, K. Inheritance Patterns, Dominance and Cross-Resistance of Cry1Ab- and Cry1Ac-Selected Ostrinia furnacalis (Guenée). Toxins 2014, 6, 2694-2707.

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