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Toxins 2014, 6(7), 2082-2096; doi:10.3390/toxins6072082

Intraspecific Variation of Centruroides Edwardsii Venom from Two Regions of Colombia

1
Facultad de Quimica Farmaceutica, Universidad de Antioquia UdeA, Carrera 53 No. 61-30, Medellín 050010, Colombia
2
Programa de Ofidismo/Escorpionismo, Facultad de Química Farmacéutica, Universidad de Antioquia UdeA, Carrera 53 No. 61-30, Medellín 050010, Colombia
3
Grupo de Investigación de Productos Naturales, Facultad de Ciencias, Universidad del Tolima, Barrio Santa Helena Parte Alta, Ibagué 731020, Colombia
4
Facultad de Medicina, Universidad Cooperativa de Colombia, Calle 50 A No. 41-20, Medellín 050010, Colombia
*
Author to whom correspondence should be addressed.
Received: 4 April 2014 / Revised: 20 May 2014 / Accepted: 6 June 2014 / Published: 14 July 2014
(This article belongs to the Section Animal Venoms)
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Abstract

We report the first description studies, partial characterization, and intraspecific difference of Centruroides edwardsii, Gervais 1843, venom. C. edwardsii from two Colombian regions (Antioquia and Tolima) were evaluated. Both venoms showed hemolytic activity, possibly dependent of enzymatic active phospholipases, and neither coagulant nor proteolytic activities were observed. Venom electrophoretic profile showed significant differences between C. edwardsii venom from both regions. A high concentration of proteins with molecular masses between 31 kDa and 97.4 kDa, and an important concentration close or below 14.4 kDa were detected. RP-HPLC retention times between 38.2 min and 42.1 min, showed bands close to 14.4 kDa, which may correspond to phospholipases. RP-HPLC venom profile showed a well conserved region in both venoms between 7 and 17 min, after this, significant differences were detected. From Tolima region venom, 50 well-defined peaks were detected, while in the Antioquia region venom, 55 well-defined peaks were detected. Larvicidal activity was only detected in the C. edwardsii venom from Antioquia. No antimicrobial activity was observed using complete venom or RP-HPLC collected fractions of both venoms. Lethally activity (carried out on female albino swiss mice) was detected at doses over 19.2 mg/kg of crude venom. Toxic effects included distress, excitability, eye irritation and secretions, hyperventilation, ataxia, paralysis, and salivation. View Full-Text
Keywords: intraspecific variation; Centruroides edwardsii; phospholipases; lethal activity; RP-HPLC intraspecific variation; Centruroides edwardsii; phospholipases; lethal activity; RP-HPLC
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Estrada-Gómez, S.; Cupitra, N.I.; Arango, W.M.; Muñoz, L.J.V. Intraspecific Variation of Centruroides Edwardsii Venom from Two Regions of Colombia. Toxins 2014, 6, 2082-2096.

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