Toxins 2014, 6(2), 624-635; doi:10.3390/toxins6020624
Article

Botulinum Neurotoxin A Complex Recognizes Host Carbohydrates through Its Hemagglutinin Component

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Received: 24 December 2013; in revised form: 15 January 2014 / Accepted: 5 February 2014 / Published: 12 February 2014
(This article belongs to the Special Issue Neurotoxins: Health Threats and Biological Tools)
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Abstract: Botulinum neurotoxins (BoNTs) are potent bacterial toxins. The high oral toxicity of BoNTs is largely attributed to the progenitor toxin complex (PTC), which is assembled from BoNT and nontoxic neurotoxin-associated proteins (NAPs) that are produced together with BoNT in bacteria. Here, we performed ex vivo studies to examine binding of the highly homogeneous recombinant NAPs to mouse small intestine. We also carried out the first comprehensive glycan array screening with the hemagglutinin (HA) component of NAPs. Our data confirmed that intestinal binding of the PTC is partly mediated by the HA moiety through multivalent interactions between HA and host carbohydrates. The specific HA-carbohydrate recognition could be inhibited by receptor-mimicking saccharides.
Keywords: Botulinum neurotoxins (BoNTs); progenitor toxin complex (PTC); hemagglutinin (HA); carbohydrate receptor; glycan array
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yao, G.; Lee, K.; Gu, S.; Lam, K.-H.; Jin, R. Botulinum Neurotoxin A Complex Recognizes Host Carbohydrates through Its Hemagglutinin Component. Toxins 2014, 6, 624-635.

AMA Style

Yao G, Lee K, Gu S, Lam K-H, Jin R. Botulinum Neurotoxin A Complex Recognizes Host Carbohydrates through Its Hemagglutinin Component. Toxins. 2014; 6(2):624-635.

Chicago/Turabian Style

Yao, Guorui; Lee, Kwangkook; Gu, Shenyan; Lam, Kwok-Ho; Jin, Rongsheng. 2014. "Botulinum Neurotoxin A Complex Recognizes Host Carbohydrates through Its Hemagglutinin Component." Toxins 6, no. 2: 624-635.


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