Next Article in Journal
Protective Effect of Two Yeast Based Feed Additives on Pigs Chronically Exposed to Deoxynivalenol and Zearalenone
Previous Article in Journal
Bacillus thuringiensis Toxins: An Overview of Their Biocidal Activity
Article Menu

Export Article

Open AccessArticle
Toxins 2014, 6(12), 3326-3335; doi:10.3390/toxins6123326

Quantitative Analysis of Cereulide Toxin from Bacillus cereus in Rice and Pasta Using Synthetic Cereulide Standard and 13C6-Cereulide Standard—A Short Validation Study

Science Department, National Food Agency, Box 622, SE-751 26 Uppsala, Sweden
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 19 September 2014 / Revised: 12 November 2014 / Accepted: 25 November 2014 / Published: 11 December 2014
(This article belongs to the Section Bacterial Toxins)
View Full-Text   |   Download PDF [340 KB, uploaded 11 December 2014]   |  

Abstract

A single laboratory validation study of a rapid and sensitive quantitative method for the analysis of cereulide toxin produced by Bacillus cereus using ultra high performance liquid chromatography-electrospray-tandem mass spectrometry is presented. The analysis of this cyclic peptide toxin was validated for pasta and rice samples using a newly presented synthetic cereulide peptide standard, together with 13C6-cereulide that previously have not been commercially available. The use of cereulide standard was also compared to the most frequently used surrogate standard, the antibiotic valinomycin. The performance of the method was evaluated by analyzing spiked sample pools from different types of rice and pasta, as well as 21 individual rice and pasta samples from differently prepared meals. Inoculation of samples with three cereulide toxin-producing strains of Bacillus cereus was finally used to mimic naturally contaminated foods. The quantification range of the method was 1–500 ng/g (R2 = 0.999) and the limits of detection and quantification were 0.1 and 1 ng/g, respectively. The precision varied from 3% to 7% relative standard deviation and the trueness from −2% to +6% relative bias at different concentration levels in cooked rice and pasta. View Full-Text
Keywords: cereulide; toxin; Bacillus cereus; validation; foods; UPLC-MS/MS cereulide; toxin; Bacillus cereus; validation; foods; UPLC-MS/MS
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zuberovic Muratovic, A.; Tröger, R.; Granelli, K.; Hellenäs, K.-E. Quantitative Analysis of Cereulide Toxin from Bacillus cereus in Rice and Pasta Using Synthetic Cereulide Standard and 13C6-Cereulide Standard—A Short Validation Study. Toxins 2014, 6, 3326-3335.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top