Quantitative Analysis of Cereulide Toxin from Bacillus cereus in Rice and Pasta Using Synthetic Cereulide Standard and 13C6-Cereulide Standard—A Short Validation Study
Abstract
:1. Introduction
2. Results and Discussion
2.1. Validation Design
2.2. Calibration and Linearity
2.3. Precision, Trueness and Recovery
Matrix | Type of sample | Spiking level (ng/g) | Found (ng/g) | Trueness (% Bias) | RSD a (%) | Recovery b (%) | Number of samples c |
---|---|---|---|---|---|---|---|
Pasta | pooled | 1.0 | 0.98 | −2.4 | 5.6 | 18 | |
pooled | 10 | 10.6 | +6.3 | 2.9 | 18 | ||
pooled | 100 | 103 | +3.5 | 3.5 | 18 | ||
individual | 5.0 | 4.92 | −1.5 | 3.3 | 91 | 21 | |
Rice | pooled | 1.0 | 0.97 | −3.4 | 6.6 | 18 | |
pooled | 10 | 10.5 | +4.7 | 4.2 | 18 | ||
pooled | 100 | 105 | +5.2 | 3.4 | 18 | ||
individual | 5.0 | 4.77 | −4.5 | 3.1 | 93 | 21 |
2.4. LOD and LOQ
Analyte | Precursor ion (m/z) | Product ion (m/z) | Cone (V) | Collision (eV) |
---|---|---|---|---|
Cereulide | 1170.7 | 357.3 | 64 | 68 |
1170.7 | 314.2 | 64 | 72 | |
1170.7 | 172.15 | 64 | 80 | |
Cereulide-C13 | 1176.7 | 315.2 | 56 | 70 |
1176.7 | 172.15 | 56 | 80 | |
1176.7 | 72.1 | 56 | 80 |
2.5. Specificity
2.6. Incurred Samples
3. Experimental Section
3.1. Chemicals and Materials
3.2. Validation Samples
3.3. Sample Extraction
3.4. UPLC-ESI-MS/MS
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Zuberovic Muratovic, A.; Tröger, R.; Granelli, K.; Hellenäs, K.-E. Quantitative Analysis of Cereulide Toxin from Bacillus cereus in Rice and Pasta Using Synthetic Cereulide Standard and 13C6-Cereulide Standard—A Short Validation Study. Toxins 2014, 6, 3326-3335. https://doi.org/10.3390/toxins6123326
Zuberovic Muratovic A, Tröger R, Granelli K, Hellenäs K-E. Quantitative Analysis of Cereulide Toxin from Bacillus cereus in Rice and Pasta Using Synthetic Cereulide Standard and 13C6-Cereulide Standard—A Short Validation Study. Toxins. 2014; 6(12):3326-3335. https://doi.org/10.3390/toxins6123326
Chicago/Turabian StyleZuberovic Muratovic, Aida, Rikard Tröger, Kristina Granelli, and Karl-Erik Hellenäs. 2014. "Quantitative Analysis of Cereulide Toxin from Bacillus cereus in Rice and Pasta Using Synthetic Cereulide Standard and 13C6-Cereulide Standard—A Short Validation Study" Toxins 6, no. 12: 3326-3335. https://doi.org/10.3390/toxins6123326