Effects of Hydrogen Peroxide and Ultrasound on Biomass Reduction and Toxin Release in the Cyanobacterium, Microcystis aeruginosa
Abstract
:1. Introduction
2. Results
2.1. Hydrogen Peroxide
H2O2 treatment | Chl-a (µg L−1) | ΦPSII (-) | Particles (No. mL−1) | |||
---|---|---|---|---|---|---|
Tukey post hoc comparison | ||||||
Control | a | a | a | |||
1 mg L−1 | a | a,b | a | |||
2 mg L−1 | a | b | a | |||
4 mg L−1 | b | c | b | |||
8 mg L−1 | c | c | b | |||
repeated measures ANOVA | F | p | F | p | F | p |
treatment effect (F4,10) | 367.5 | <0.001 | 284.1 | <0.001 | 102.4 | <0.001 |
time effect (F3,30) | 695.3 | <0.001 | 145.5 | <0.001 | 80.0 | <0.001 |
time × treatment interaction (F12,30) | 215.0 | <0.001 | 46.5 | <0.001 | 41.1 | <0.001 |
nonlinear regression | r2 adj | r2 adj | r2 adj | |||
EC50 (mg H2O2 L−1) | 3.8 | 0.992 | 2.5 | 0.947 | 2.6 | 0.929 |
2.2. Ultrasound
Treatments | Chl-a(µg L−1) | ΦPSII (-) | Particles (# mL−1) | |||
---|---|---|---|---|---|---|
Tukey post hoc comparison | ||||||
Control | a | a | a | |||
AL-05 | a | b | b | |||
AL-10 | a | b | b | |||
repeated measures ANOVA | F | p | F | p | F | p |
treatment effect (F2,6) | 3.84 | 0.084 | 64.5 | <0.001 | 200.9 | <0.001 |
time effect (F5,30) | 423.3 | <0.001 | 2.35 | 0.065 | 103.8 | <0.001 |
time × treatment interaction (F10,30) | 5.61 | <0.001 | 1.56 | 0.169 | 42.9 | <0.001 |
3. Discussion
3.1. Hydrogen Peroxide
MC-variant | Our study | Ríos et al. [46] | Robillot et al. [44] |
---|---|---|---|
MC-LR | 81.2 | 79.5 | 51.0 |
MC-LY | 2.2 | 1.6 | 8.6 |
MC-LF | 8.0 | 0.6 | 19.4 |
MC-LW | 5.8 | 1.0 | 21.0 |
others | 2.8 | 17.3 | - |
3.2. Ultrasound
3.3. Overall
4. Experimental Section
4.1. Organisms
4.2. Hydrogen Peroxide
4.3. Ultrasound
4.4. MC Analysis
4.5. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Lürling, M.; Meng, D.; Faassen, E.J. Effects of Hydrogen Peroxide and Ultrasound on Biomass Reduction and Toxin Release in the Cyanobacterium, Microcystis aeruginosa. Toxins 2014, 6, 3260-3280. https://doi.org/10.3390/toxins6123260
Lürling M, Meng D, Faassen EJ. Effects of Hydrogen Peroxide and Ultrasound on Biomass Reduction and Toxin Release in the Cyanobacterium, Microcystis aeruginosa. Toxins. 2014; 6(12):3260-3280. https://doi.org/10.3390/toxins6123260
Chicago/Turabian StyleLürling, Miquel, Debin Meng, and Elisabeth J. Faassen. 2014. "Effects of Hydrogen Peroxide and Ultrasound on Biomass Reduction and Toxin Release in the Cyanobacterium, Microcystis aeruginosa" Toxins 6, no. 12: 3260-3280. https://doi.org/10.3390/toxins6123260