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Toxins 2014, 6(11), 3144-3156; doi:10.3390/toxins6113144

Molecular and Insecticidal Characterization of a Novel Cry-Related Protein from Bacillus Thuringiensis Toxic against Myzus persicae

1
Instituto de Agrobiotecnología, CSIC-UPNA-Gobierno de Navarra, Campus Arrosadía, Mutilva 31192, Navarra, Spain
2
Grupo de Protección Cultivos, Departamento de Producción Agraria, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Pública de Navarra, Pamplona 31006, Navarra, Spain
3
Cardiff School of Biosciences, Cardiff University, Park Place, Cardiff CF10 3AT, UK
*
Author to whom correspondence should be addressed.
Received: 15 October 2014 / Revised: 28 October 2014 / Accepted: 28 October 2014 / Published: 6 November 2014
(This article belongs to the Section Bacterial Toxins)
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Abstract

This study describes the insecticidal activity of a novel Bacillus thuringiensis Cry-related protein with a deduced 799 amino acid sequence (~89 kDa) and ~19% pairwise identity to the 95-kDa-aphidicidal protein (sequence number 204) from patent US 8318900 and ~40% pairwise identity to the cancer cell killing Cry proteins (parasporins Cry41Ab1 and Cry41Aa1), respectively. This novel Cry-related protein contained the five conserved amino acid blocks and the three conserved domains commonly found in 3-domain Cry proteins. The protein exhibited toxic activity against the green peach aphid, Myzus persicae (Sulzer) (Homoptera: Aphididae) with the lowest mean lethal concentration (LC50 = 32.7 μg/mL) reported to date for a given Cry protein and this insect species, whereas it had no lethal toxicity against the Lepidoptera of the family Noctuidae Helicoverpa armigera (Hübner), Mamestra brassicae (L.), Spodoptera exigua (Hübner), S. frugiperda (J.E. Smith) and S. littoralis (Boisduval), at concentrations as high as ~3.5 μg/cm2. This novel Cry-related protein may become a promising environmentally friendly tool for the biological control of M. persicae and possibly also for other sap sucking insect pests. View Full-Text
Keywords: Bacillus thuringiensis; δ-endotoxins; Cry-related protein; parasporins; green peach aphid; Myzus persicae Bacillus thuringiensis; δ-endotoxins; Cry-related protein; parasporins; green peach aphid; Myzus persicae
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Palma, L.; Muñoz, D.; Berry, C.; Murillo, J.; de Escudero, I.R.; Caballero, P. Molecular and Insecticidal Characterization of a Novel Cry-Related Protein from Bacillus Thuringiensis Toxic against Myzus persicae. Toxins 2014, 6, 3144-3156.

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