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Toxins 2012, 4(10), 768-777; doi:10.3390/toxins4100768
Article

Antibody to Heat Shock Protein 70 (HSP70) Inhibits Human T-Cell Lymphoptropic Virus Type I (HTLV-I) Production by Transformed Rabbit T-Cell Lines

1
 and 2,*
Received: 11 June 2012; in revised form: 12 September 2012 / Accepted: 14 September 2012 / Published: 26 September 2012
(This article belongs to the collection Toxicity and Therapeutic Interventions in the Immune System)
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Abstract: Adult T cell leukemia is a fatal malignant transformation caused by the human T-cell lymphoptropic virus type I (HTLV-I). HTLV-I is only associated with the development of this disease in a small percentage of infected individuals. Using two rabbit transformed T-cell lines; RH/K30 (asymptomatic) and RH/K34 (leukemogenic), we have investigated the expression of heat shock proteins (HSP) 90 and 70 and the role of anti-HSPs antibodies on virus production. HSPs surface expression was higher on RH/K34 than RH/K30 cells. Heat treatment of cells increased the expression of HSPs proteins and virus production; HSPs augmentation was stabilized after 12 h and virus production reached a maximum between 8 h–12 h then returned to normal level after 24 h of culture. Incubation of cells only with rabbit anti-HSP 70 antibodies prevented virus production specifically in the leukemogenic cell line. The results indicate a relationship between HSP 70 and virus production.
Keywords: HTLV-I; leukemogenesis; heat shock proteins; antibodies; animal model HTLV-I; leukemogenesis; heat shock proteins; antibodies; animal model
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Fallouh, H.; Mahana, W. Antibody to Heat Shock Protein 70 (HSP70) Inhibits Human T-Cell Lymphoptropic Virus Type I (HTLV-I) Production by Transformed Rabbit T-Cell Lines. Toxins 2012, 4, 768-777.

AMA Style

Fallouh H, Mahana W. Antibody to Heat Shock Protein 70 (HSP70) Inhibits Human T-Cell Lymphoptropic Virus Type I (HTLV-I) Production by Transformed Rabbit T-Cell Lines. Toxins. 2012; 4(10):768-777.

Chicago/Turabian Style

Fallouh, Hanan; Mahana, Wahib. 2012. "Antibody to Heat Shock Protein 70 (HSP70) Inhibits Human T-Cell Lymphoptropic Virus Type I (HTLV-I) Production by Transformed Rabbit T-Cell Lines." Toxins 4, no. 10: 768-777.



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