Next Article in Journal
Incidence of Fusarium Species and Mycotoxins in Silage Maize
Previous Article in Journal / Special Issue
Spatial Patterns of Aflatoxin Levels in Relation to Ear-Feeding Insect Damage in Pre-Harvest Corn
Article Menu

Export Article

Open AccessArticle
Toxins 2011, 3(8), 932-948; doi:10.3390/toxins3080932

Expression Profiling of Non-Aflatoxigenic Aspergillus parasiticus Mutants Obtained by 5-Azacytosine Treatment or Serial Mycelial Transfer

1
Southern Regional Research Center, ARS/USDA, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, USA
2
Department of Biology, 1 Drexel Dr., Box 85B, Xavier University of Louisiana, New Orleans, LA 70125, USA
*
Author to whom correspondence should be addressed.
Received: 25 May 2011 / Revised: 19 July 2011 / Accepted: 26 July 2011 / Published: 2 August 2011
(This article belongs to the Special Issue Aflatoxins 2011)
View Full-Text   |   Download PDF [210 KB, uploaded 2 August 2011]   |  

Abstract

Aflatoxins are carcinogenic secondary metabolites produced by the fungi Aspergillus flavus and Aspergillus parasiticus. Previous studies found that repeated serial mycelial transfer or treatment of A. parasiticus with 5-azacytidine produced colonies with a fluffy phenotype and inability to produce aflatoxins. To understand how these treatments affect expression of genes involved in aflatoxin production and development, we carried out expressed sequence tag (EST)-based microarray assays to identify genes in treated clones that are differentially expressed compared to the wild-type. Expression of 183 genes was significantly dysregulated. Of these, 38 had at least two-fold or lower expression compared to the untreated control and only two had two-fold or higher expression. The most frequent change was downregulation of genes predicted to encode membrane-bound proteins. Based on this result we hypothesize that the treatments cause changes in the structure of cellular and organelle membranes that prevent normal development and aflatoxin biosynthesis.
Keywords: aflatoxin; microarray; 5-azacytidine; Aspergillus parasiticus; secondary metabolism; fluffy phenotype aflatoxin; microarray; 5-azacytidine; Aspergillus parasiticus; secondary metabolism; fluffy phenotype
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wilkinson, J.R.; Kale, S.P.; Bhatnagar, D.; Yu, J.; Ehrlich, K.C. Expression Profiling of Non-Aflatoxigenic Aspergillus parasiticus Mutants Obtained by 5-Azacytosine Treatment or Serial Mycelial Transfer. Toxins 2011, 3, 932-948.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top