Toxins 2011, 3(7), 766-786; doi:10.3390/toxins3070766

Transcriptional Profiles Uncover Aspergillus flavus-Induced Resistance in Maize Kernels

1email, 2,* email, 1email, 3email, 2email and 2email
Received: 15 April 2011; in revised form: 23 June 2011 / Accepted: 23 June 2011 / Published: 29 June 2011
(This article belongs to the Special Issue Aflatoxins)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Aflatoxin contamination caused by the opportunistic pathogen A. flavus is a major concern in maize production prior to harvest and through storage. Previous studies have highlighted the constitutive production of proteins involved in maize kernel resistance against A. flavus’ infection. However, little is known about induced resistance nor about defense gene expression and regulation in kernels. In this study, maize oligonucleotide arrays and a pair of closely-related maize lines varying in aflatoxin accumulation were used to reveal the gene expression network in imbibed mature kernels in response to A. flavus’ challenge. Inoculated kernels were incubated 72 h via the laboratory-based Kernel Screening Assay (KSA), which highlights kernel responses to fungal challenge. Gene expression profiling detected 6955 genes in resistant and 6565 genes in susceptible controls; 214 genes induced in resistant and 2159 genes induced in susceptible inoculated kernels. Defense related and regulation related genes were identified in both treatments. Comparisons between the resistant and susceptible lines indicate differences in the gene expression network which may enhance our understanding of the maize-A. flavus interaction.
Keywords: Zea mays; Aspergillus flavus; imbibed kernels; aflatoxin; resistance genes
PDF Full-text Download PDF Full-Text [398 KB, Updated Version, uploaded 11 July 2011 14:48 CEST]
The original version is still available [487 KB, uploaded 29 June 2011 14:37 CEST]

Export to BibTeX |

MDPI and ACS Style

Luo, M.; Brown, R.L.; Chen, Z.-Y.; Menkir, A.; Yu, J.; Bhatnagar, D. Transcriptional Profiles Uncover Aspergillus flavus-Induced Resistance in Maize Kernels. Toxins 2011, 3, 766-786.

AMA Style

Luo M, Brown RL, Chen Z-Y, Menkir A, Yu J, Bhatnagar D. Transcriptional Profiles Uncover Aspergillus flavus-Induced Resistance in Maize Kernels. Toxins. 2011; 3(7):766-786.

Chicago/Turabian Style

Luo, Meng; Brown, Robert L.; Chen, Zhi-Yuan; Menkir, Abebe; Yu, Jiujiang; Bhatnagar, Deepak. 2011. "Transcriptional Profiles Uncover Aspergillus flavus-Induced Resistance in Maize Kernels." Toxins 3, no. 7: 766-786.

Toxins EISSN 2072-6651 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert