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Toxins 2010, 2(4), 382-398; doi:10.3390/toxins2040382
Article

Development of an Electrochemical Immunosensor for Fumonisins Detection in Foods

 and
*
Cranfield University, Cranfield Health, Cranfield, Bedfordshire, MK43 0AL, UK
* Author to whom correspondence should be addressed.
Received: 29 January 2010 / Revised: 23 February 2010 / Accepted: 19 March 2010 / Published: 24 March 2010
(This article belongs to the Special Issue Advances in Mycotoxin Research)
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Abstract

An electrochemical affinity sensor for the determination of fumonisins mycotoxins (Fms) using monoclonal antibody modified screen-printed gold electrode with carbon counter and silver-silver chloride pseudo-reference electrode is reported in this work. A direct competitive enzyme-linked immunosorbent assay (ELISA) was initially developed, exhibiting a detection limit of 100 µg·L-1for fumonisins. This was then transferred to the surface of a bare gold screen-printed electrode (SPGE) and detection was performed by chronoamperometry, monitoring the reaction of 3,3’,5,5’-Tetramethylbenzidine dihydrochloride (TMB) and hydrogen peroxide (H2O2) catalysed by HRP at −100 mV potential vs. onboard Ag-AgCl pseudo-reference electrode. The immunosensor exhibited detection limit of 5 µg·L−1 fumonisins with a dynamic range from 1 µg·L−1–1000 µg·L−1. The sensor also performed well in extracted corn samples.
Keywords: fumonisins; mycotoxins; screen-printed gold electrode; electrochemical immunosensor fumonisins; mycotoxins; screen-printed gold electrode; electrochemical immunosensor
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Abdul Kadir, M.K.; Tothill, I.E. Development of an Electrochemical Immunosensor for Fumonisins Detection in Foods. Toxins 2010, 2, 382-398.

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