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Toxins 2010, 2(4), 382-398; doi:10.3390/toxins2040382
Article

Development of an Electrochemical Immunosensor for Fumonisins Detection in Foods

 and *
Received: 29 January 2010; in revised form: 23 February 2010 / Accepted: 19 March 2010 / Published: 24 March 2010
(This article belongs to the Special Issue Advances in Mycotoxin Research)
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Abstract: An electrochemical affinity sensor for the determination of fumonisins mycotoxins (Fms) using monoclonal antibody modified screen-printed gold electrode with carbon counter and silver-silver chloride pseudo-reference electrode is reported in this work. A direct competitive enzyme-linked immunosorbent assay (ELISA) was initially developed, exhibiting a detection limit of 100 µg·L-1for fumonisins. This was then transferred to the surface of a bare gold screen-printed electrode (SPGE) and detection was performed by chronoamperometry, monitoring the reaction of 3,3’,5,5’-Tetramethylbenzidine dihydrochloride (TMB) and hydrogen peroxide (H2O2) catalysed by HRP at −100 mV potential vs. onboard Ag-AgCl pseudo-reference electrode. The immunosensor exhibited detection limit of 5 µg·L−1 fumonisins with a dynamic range from 1 µg·L−1–1000 µg·L−1. The sensor also performed well in extracted corn samples.
Keywords: fumonisins; mycotoxins; screen-printed gold electrode; electrochemical immunosensor fumonisins; mycotoxins; screen-printed gold electrode; electrochemical immunosensor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Abdul Kadir, M.K.; Tothill, I.E. Development of an Electrochemical Immunosensor for Fumonisins Detection in Foods. Toxins 2010, 2, 382-398.

AMA Style

Abdul Kadir MK, Tothill IE. Development of an Electrochemical Immunosensor for Fumonisins Detection in Foods. Toxins. 2010; 2(4):382-398.

Chicago/Turabian Style

Abdul Kadir, Mohamad Kamal; Tothill, Ibtisam E. 2010. "Development of an Electrochemical Immunosensor for Fumonisins Detection in Foods." Toxins 2, no. 4: 382-398.


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