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Toxins 2018, 10(8), 332; https://doi.org/10.3390/toxins10080332

Purification and Characterization of a Novel Antiplatelet Peptide from Deinagkistrodon acutus Venom

School of Life Science and Technology, China Pharmaceutical University, 24 Tong Jia Street, Nanjing 210009, China
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Received: 30 June 2018 / Revised: 13 August 2018 / Accepted: 14 August 2018 / Published: 16 August 2018
(This article belongs to the Section Animal Venoms)
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Abstract

Animal venoms are considered as one of the most important sources for drug development. Deinagkistrodon acutus is famous for its toxicity to the human hematological system and envenomed patients develop a coagulation disorder with the symptoms of hemorrhage and microthrombi formation. The purpose of this study was to separate antiplatelet peptides from D. acutus venom using a combination of an ultrafiltration technique and reversed-phase high performance liquid chromatography (HPLC), which was guided by monitoring antiplatelet aggregation bioactivity. A novel octa-peptide named DAA-8 was found. This peptide inhibited protease-activated receptor1 (PAR-1) agonist (SFLLRN-NH2) induced platelet aggregation and it also inhibited platelet aggregation induced by thrombin, ADP, and collagen. Furthermore, DAA-8 showed significant antithrombotic activity and resulted in a slightly increased bleeding risk in vivo. This is the first report of a peptide derived from snake venom, which inhibited PAR-1 agonist-induced platelet aggregation. This peptide may provide a template to design a new PAR-1 inhibitor. View Full-Text
Keywords: Deinagkistrodon acutus; DAA-8; antiplatelet; PAR-1 Deinagkistrodon acutus; DAA-8; antiplatelet; PAR-1
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Kong, Y.; Sun, Q.; Zhao, Q.; Zhang, Y. Purification and Characterization of a Novel Antiplatelet Peptide from Deinagkistrodon acutus Venom. Toxins 2018, 10, 332.

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