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Toxins 2018, 10(4), 165; https://doi.org/10.3390/toxins10040165

Visual Non-Instrumental On-Site Detection of Fumonisin B1, B2, and B3 in Cereal Samples Using a Clean-Up Combined with Gel-Based Immunoaffinity Test Column Assay

1
State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China
2
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, China
*
Authors to whom correspondence should be addressed.
Received: 13 March 2018 / Revised: 15 April 2018 / Accepted: 17 April 2018 / Published: 19 April 2018
(This article belongs to the Collection Biorecognition Assays for Mycotoxins)
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Abstract

A visual immunoaffinity test column (IATC) assay was developed to detect fumonisins in cereal samples for spot tests without the need for special instruments. The developed IATC assay had equivalent recognition capability for fumonisin B1 (FB1), fumonisin B2 (FB2), or fumonisin B3 (FB3), and exhibited no cross-reactivity with aflatoxin B1, ochratoxin A, zearalenone, or the T-2 toxin. The sample pretreatment was accomplished more rapidly and with greater ease, the entire assay procedure was completed in approximately 10 min, including sample pretreatment and testing. The limits of detection (LODs) of the IATC assay to detect fumonisins in the maize, barley, oat, and millet samples were 20 μg kg−1. The results of the spiked maize, barley, oat, and millet and real maize samples by the IATC assay agreed well with the results obtained by the commercial fumonisin enzyme-linked immunosorbent assay (ELISA) test kit and liquid chromatography-tandem mass spectrometry (LC-MS/MS), respectively. The developed IATC assay can serve as a useful screening tool for the rapid, qualitative, and semi-quantitative detection of the total content of fumonisins (sum of FB1, FB2, and FB3) in cereal samples on-site. View Full-Text
Keywords: fumonisins; immunoaffinity test column; clean-up column; visual non-instrumental detection; cereal samples fumonisins; immunoaffinity test column; clean-up column; visual non-instrumental detection; cereal samples
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Sheng, W.; Wu, H.; Ji, W.; Li, Z.; Chu, F.; Wang, S. Visual Non-Instrumental On-Site Detection of Fumonisin B1, B2, and B3 in Cereal Samples Using a Clean-Up Combined with Gel-Based Immunoaffinity Test Column Assay. Toxins 2018, 10, 165.

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