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Toxins 2018, 10(5), 180; https://doi.org/10.3390/toxins10050180

Nanobody Technology for Mycotoxin Detection: Current Status and Prospects

1
State Key Laboratory of Magnetic Resonance and Atomic Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China
2
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
*
Authors to whom correspondence should be addressed.
Received: 10 April 2018 / Revised: 24 April 2018 / Accepted: 27 April 2018 / Published: 29 April 2018
(This article belongs to the Collection Biorecognition Assays for Mycotoxins)
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Abstract

Mycotoxins, which are toxic, carcinogenic, and/or teratogenic, have posed a threat to food safety and public health. Sensitive and effective determination technologies for mycotoxin surveillance are required. Immunoassays have been regarded as useful supplements to chromatographic techniques. However, conventional antibodies involved in immunoassays are difficult to be expressed recombinantly and are susceptible to harsh environments. Nanobodies (or VHH antibodies) are antigen-binding sites of the heavy-chain antibodies produced from Camelidae. They are found to be expressed easily in prokaryotic or eukaryotic expression systems, more robust in extreme conditions, and facile to be used as surrogates for artificial antigens. These properties make them the promising and environmentally friendly immunoreagents in the next generation of immunoassays. This review briefly describes the latest developments in the area of nanobodies used in mycotoxin detection. Moreover, by integrating the introduction of the principle of nanobodies production and the critical assessment of their performance, this paper also proposes the prospect of nanobodies in the field of food safety in the foreseeable future. View Full-Text
Keywords: Nanobody; Mycotoxins; Immunoassay; Food safety Nanobody; Mycotoxins; Immunoassay; Food safety
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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He, T.; Zhu, J.; Nie, Y.; Hu, R.; Wang, T.; Li, P.; Zhang, Q.; Yang, Y. Nanobody Technology for Mycotoxin Detection: Current Status and Prospects. Toxins 2018, 10, 180.

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