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Toxins 2018, 10(3), 99; doi:10.3390/toxins10030099

Proteomic Methods of Detection and Quantification of Protein Toxins

Faculty of Military Health Sciences, University of Defense in Brno, Třebešská 1575, CZ-500 01 Hradec Králové, Czech Republic
Military Health Institute, Military Medical Agency, Tychonova 1, CZ-160 00 Prague 6, Czech Republic
Author to whom correspondence should be addressed.
Received: 12 February 2018 / Revised: 21 February 2018 / Accepted: 23 February 2018 / Published: 28 February 2018
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Biological toxins are a heterogeneous group of compounds that share commonalities with biological and chemical agents. Among them, protein toxins represent a considerable, diverse set. They cover a broad range of molecular weights from less than 1000 Da to more than 150 kDa. This review aims to compare conventional detection methods of protein toxins such as in vitro bioassays with proteomic methods, including immunoassays and mass spectrometry-based techniques and their combination. Special emphasis is given to toxins falling into a group of selected agents, according to the Centers for Disease Control and Prevention, such as Staphylococcal enterotoxins, Bacillus anthracis toxins, Clostridium botulinum toxins, Clostridium perfringens epsilon toxin, ricin from Ricinus communis, Abrin from Abrus precatorius or control of trade in dual-use items in the European Union, including lesser known protein toxins such as Viscumin from Viscum album. The analysis of protein toxins and monitoring for biological threats, i.e., the deliberate spread of infectious microorganisms or toxins through water, food, or the air, requires rapid and reliable methods for the early identification of these agents. View Full-Text
Keywords: protein toxins; analytical methods; proteomic; bio-terrorism protein toxins; analytical methods; proteomic; bio-terrorism

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Duracova, M.; Klimentova, J.; Fucikova, A.; Dresler, J. Proteomic Methods of Detection and Quantification of Protein Toxins. Toxins 2018, 10, 99.

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