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Toxins 2018, 10(2), 87; https://doi.org/10.3390/toxins10020087

An On-Site Simultaneous Semi-Quantification of Aflatoxin B1, Zearalenone, and T-2 Toxin in Maize- and Cereal-Based Feed via Multicolor Immunochromatographic Assay

1,2,3,4,†
,
1,3,†,* , 1,2,3,* , 1,2,3
,
1,2
,
1,2
,
1,4
and
1,2,3,4,*
1
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
2
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China
3
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China
4
Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 9 January 2018 / Revised: 8 February 2018 / Accepted: 15 February 2018 / Published: 17 February 2018
(This article belongs to the Special Issue Advanced Sensors for Toxins)
View Full-Text   |   Download PDF [1366 KB, uploaded 23 February 2018]   |  

Abstract

Multiple-mycotoxin contamination has been frequently found in the agro-food monitoring due to the coexistence of fungi. However, many determination methods focused on a single mycotoxin, highlighting the demand for on-site determination of multiple mycotoxins in a single run. We develop a multicolor-based immunochromatographic strip (ICS) for simultaneous determination of aflatoxin B1 (AFB1), zearalenone (ZEN) and T-2 toxin in maize- and cereal-based animal feeds. The nanoparticles with different colors are conjugated with three monoclonal antibodies, which serve as the immunoassay probes. The decrease in color intensity is observed by the naked eyes, providing simultaneous quantification of three mycotoxins. The visible limits of detection for AFB1, ZEN and T-2 are estimated to be 0.5, 2, and 30 ng/mL, respectively. The cut-off values are 1, 10, and 50 ng/mL, respectively. Considerable specificity and stability are found using real samples. The results are in excellent agreement with those from high-performance liquid chromatography/tandem mass spectrometry. The multi-color ICS boasts sensitive and rapid visual differentiation and simultaneous semi-quantification of aflatoxin B1, zearalenone and T-2 toxin in maize- and cereal-based feed samples within 20 min. View Full-Text
Keywords: multicolor; immunochromatographic assay; aflatoxin B1; zearalenone; T-2 multicolor; immunochromatographic assay; aflatoxin B1; zearalenone; T-2
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Xu, L.; Zhang, Z.; Zhang, Q.; Zhang, W.; Yu, L.; Wang, D.; Li, H.; Li, P. An On-Site Simultaneous Semi-Quantification of Aflatoxin B1, Zearalenone, and T-2 Toxin in Maize- and Cereal-Based Feed via Multicolor Immunochromatographic Assay. Toxins 2018, 10, 87.

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