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Nutrients 2017, 9(9), 969; doi:10.3390/nu9090969

Simulation of Food Folate Digestion and Bioavailability of an Oxidation Product of 5-Methyltetrahydrofolate

1
Division BIOANALYTIK Weihenstephan, Research Center for Nutrition and Food Sciences (Z. I. E. L.), Technical University of Munich, Alte Akademie 10, D-85350 Freising, Germany
2
Chair of Analytical Food Chemistry, Technical University of Munich, Alte Akademie 10, D-85350 Freising, Germany
*
Author to whom correspondence should be addressed.
Received: 22 July 2017 / Revised: 26 August 2017 / Accepted: 28 August 2017 / Published: 1 September 2017
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Abstract

Generating bioavailability data from in vivo studies is time-consuming and expensive. In vitro simulation can help to investigate factors influencing bioavailability or facilitate quantifying the impact of such factors. For folates, an efficient deconjugation of polyglutamates to the corresponding monoglutamates is crucial for bioavailability and highly dependent on the food matrix. Therefore, the bioaccessibility of folates of different foodstuffs was examined using a simulated digestion model with respect to folate stability and the efficiency of deconjugation. For realistic simulated deconjugation, porcine brush border membrane was used during the phase of the simulated digestion in the small intestine. For a better understanding of folate behaviour during digestion, single folate monoglutamates were also investigated with this in vitro digestion model. The results for bioaccessibility were compared with data from a human bioavailability study. They support the idea that both stability and deconjugation have an influence on bioaccessibility and thus on bioavailability. Tetrahydrofolate is probably lost completely or at least to a high extent and the stability of 5-methyltetrahydrofolate depends on the food matrix. Additionally, 5-methyltetrahydrofolate can be oxidised to a pyrazino-s-triazine (MeFox), whose absorption in the human intestinal tract was shown tentatively. View Full-Text
Keywords: LC-MS/MS; food folate; stable isotope dilution assay; in vitro simulation; bioaccessibility; oxidation product of 5-CH3-H4folate; MeFox LC-MS/MS; food folate; stable isotope dilution assay; in vitro simulation; bioaccessibility; oxidation product of 5-CH3-H4folate; MeFox
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Ringling, C.; Rychlik, M. Simulation of Food Folate Digestion and Bioavailability of an Oxidation Product of 5-Methyltetrahydrofolate. Nutrients 2017, 9, 969.

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