Eicosapentaenoic Acid (EPA) Induced Macrophages Activation through GPR120-Mediated Raf-ERK1/2-IKKβ-NF-κB p65 Signaling Pathways
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cell Culture
2.3. Cell Proliferation Assay
2.4. Morphologic Observations
2.5. Measurement of NO Production
2.6. Cytokine Assays
2.7. Nuclear Protein Extraction
2.8. Western Blot Analysis
2.9. Immunofluorescence Assay for NF-κB p65
2.10. Statistical Analysis
3. Result
3.1. EPA Increased the Proliferation Index of RAW264.7 Cells
3.2. EPA Induced the Morphological Changes of RAW264.7 Cells
3.3. EPA Induced NO Production of RAW264.7 Cells
3.4. Effect of EPA on GPR120 Protein Expression
3.5. Measurement of ERK, Raf and IKKβ
3.6. Relationship between ERK1/2 and Raf, IKKβ
3.7. Effect of EPA on the Expression of NF-κB p65
3.8. EPA Regulated the Protein Expression of iNOS and Cytokines
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Han, L.; Song, S.; Niu, Y.; Meng, M.; Wang, C. Eicosapentaenoic Acid (EPA) Induced Macrophages Activation through GPR120-Mediated Raf-ERK1/2-IKKβ-NF-κB p65 Signaling Pathways. Nutrients 2017, 9, 937. https://doi.org/10.3390/nu9090937
Han L, Song S, Niu Y, Meng M, Wang C. Eicosapentaenoic Acid (EPA) Induced Macrophages Activation through GPR120-Mediated Raf-ERK1/2-IKKβ-NF-κB p65 Signaling Pathways. Nutrients. 2017; 9(9):937. https://doi.org/10.3390/nu9090937
Chicago/Turabian StyleHan, Lirong, Shumin Song, Yabing Niu, Meng Meng, and Chunling Wang. 2017. "Eicosapentaenoic Acid (EPA) Induced Macrophages Activation through GPR120-Mediated Raf-ERK1/2-IKKβ-NF-κB p65 Signaling Pathways" Nutrients 9, no. 9: 937. https://doi.org/10.3390/nu9090937