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Nutrients 2017, 9(10), 1152; doi:10.3390/nu9101152

Comparison of the Micellar Incorporation and the Intestinal Cell Uptake of Cholecalciferol, 25-Hydroxycholecalciferol and 1-α-Hydroxycholecalciferol

1
NORT Nutrition, Obesity and Risk of Thrombosis, Aix-Marseille University, INRA, INSERM, 13385 Marseille, France
2
Adisseo France S.A.S., Center of Expertise and Research in Nutrition, 03600 Commentry, France
3
Institut Galien Paris Sud, UMR 8612, Univ Paris-Sud, CNRS, Université Paris-Saclay, 5 rue J.B. Clément, F-92296 Châtenay-Malabry, France
*
Author to whom correspondence should be addressed.
Received: 11 September 2017 / Revised: 29 September 2017 / Accepted: 11 October 2017 / Published: 23 October 2017
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Abstract

In the context of the global prevalence of vitamin D insufficiency, we compared two key determinants of the bioavailability of 3 vitamin D forms with significant biopotencies: cholecalciferol, 25-hydroxycholecalciferol and 1-α-hydroxycholecalciferol. To this aim, we studied their incorporation into synthetic mixed micelles and their uptake by intestinal cells in culture. Our results show that 1-α-hydroxycholecalciferol was significantly more solubilized into mixed micelles compared to the other forms (1.6-fold and 2.9-fold improvement compared to cholecalciferol and 25-hydroxycholecalciferol, respectively). In Caco-2 TC7 cells, the hydroxylated forms were taken up more efficiently than cholecalciferol (p < 0.05), and conversely to cholecalciferol, their uptake was neither SR-BI(Scavenger-Receptor class B type I)- nor NPC1L1 (NPC1 like intracellular cholesterol transporter 1)-dependent. Besides, the apical membrane sodium–bile acid transporter ASBT (Apical Sodium-dependent Bile acid Transporter) was not involved, at least in vitro, in the uptake of any of the three vitamin D forms. Further investigations are needed to identify the uptake pathways of both 1-α-hydroxycholecalciferol and 25-hydroxycholecalciferol. However, considering its high bioavailability, our results suggest the potential interest of using 1-α-hydroxycholecalciferol in the treatment of severe vitamin D deficiency. View Full-Text
Keywords: vitamin D; SR-BI; NPC1L1; ASBT; bioavailability; absorption; micelles; Caco-2 cells vitamin D; SR-BI; NPC1L1; ASBT; bioavailability; absorption; micelles; Caco-2 cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Desmarchelier, C.; Margier, M.; Prévéraud, D.P.; Nowicki, M.; Rosilio, V.; Borel, P.; Reboul, E. Comparison of the Micellar Incorporation and the Intestinal Cell Uptake of Cholecalciferol, 25-Hydroxycholecalciferol and 1-α-Hydroxycholecalciferol. Nutrients 2017, 9, 1152.

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