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Nutrients 2016, 8(5), 277;

A Mixed Flavonoid-Fish Oil Supplement Induces Immune-Enhancing and Anti-Inflammatory Transcriptomic Changes in Adult Obese and Overweight Women—A Randomized Controlled Trial

Department of Nutrition, School of Medicine—WG 48, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
Human Performance Laboratory, North Carolina Research Campus, Appalachian State University, 600 Laureate Way, Kannapolis, NC 28081, USA
Levine Center for Health and Wellness, Queens University of Charlotte, 1900 Selwyn Avenue, Charlotte, NC 28274, USA
Department of Health & Exercise Science, Appalachian State University, ASU Box 32071, 111 Rivers Street, 050 Convocation Center, Boone, NC 28608, USA
North Carolina Research Campus, Dole Nutrition Research Laboratory, Kannapolis, NC 28081, USA
Bioinformatics Services Division, North Carolina Research Campus, UNC Charlotte, 600 Laureate Way, Kannapolis, NC 28081, USA
Program in Cardiovascular & Metabolic Diseases and Center for Computational Biology, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore
Author to whom correspondence should be addressed.
Received: 5 February 2016 / Revised: 13 April 2016 / Accepted: 19 April 2016 / Published: 11 May 2016
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Flavonoids and fish oils have anti-inflammatory and immune-modulating influences. The purpose of this study was to determine if a mixed flavonoid-fish oil supplement (Q-Mix; 1000 mg quercetin, 400 mg isoquercetin, 120 mg epigallocatechin (EGCG) from green tea extract, 400 mg n3-PUFAs (omega-3 polyunsaturated fatty acid) (220 mg eicosapentaenoic acid (EPA) and 180 mg docosahexaenoic acid (DHA)) from fish oil, 1000 mg vitamin C, 40 mg niacinamide, and 800 µg folic acid) would reduce complications associated with obesity; that is, reduce inflammatory and oxidative stress markers and alter genomic profiles in overweight women. Overweight and obese women (n = 48; age = 40–70 years) were assigned to Q-Mix or placebo groups using randomized double-blinded placebo-controlled procedures. Overnight fasted blood samples were collected at 0 and 10 weeks and analyzed for cytokines, C-reactive protein (CRP), F2-isoprostanes, and whole-blood-derived mRNA, which was assessed using Affymetrix HuGene-1_1 ST arrays. Statistical analysis included two-way ANOVA models for blood analytes and gene expression and pathway and network enrichment methods for gene expression. Plasma levels increased with Q-Mix supplementation by 388% for quercetin, 95% for EPA, 18% for DHA, and 20% for docosapentaenoic acid (DPA). Q-Mix did not alter plasma levels for CRP (p = 0.268), F2-isoprostanes (p = 0.273), and cytokines (p > 0.05). Gene set enrichment analysis revealed upregulation of pathways in Q-Mix vs. placebo related to interferon-induced antiviral mechanism (false discovery rate, FDR < 0.001). Overrepresentation analysis further disclosed an inhibition of phagocytosis-related inflammatory pathways in Q-Mix vs. placebo. Thus, a 10-week Q-Mix supplementation elicited a significant rise in plasma quercetin, EPA, DHA, and DPA, as well as stimulated an antiviral and inflammation whole-blood transcriptomic response in overweight women. View Full-Text
Keywords: dietary supplement; flavonol; genomic profiling; omega-3; quercetin dietary supplement; flavonol; genomic profiling; omega-3; quercetin

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Cialdella-Kam, L.; Nieman, D.C.; Knab, A.M.; Shanely, R.A.; Meaney, M.P.; Jin, F.; Sha, W.; Ghosh, S. A Mixed Flavonoid-Fish Oil Supplement Induces Immune-Enhancing and Anti-Inflammatory Transcriptomic Changes in Adult Obese and Overweight Women—A Randomized Controlled Trial. Nutrients 2016, 8, 277.

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