Nutrients 2016, 8(10), 599; doi:10.3390/nu8100599
The Growth of SGC-7901 Tumor Xenografts Was Suppressed by Chinese Bayberry Anthocyanin Extract through Upregulating KLF6 Gene Expression
1
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China
2
Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China
3
Taizhou Academy of Agricultural Sciences, Linhai 317000, China
4
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
5
National Light Industry Food Quality Inspection Hangzhou Station, Hangzhou 310009, China
*
Author to whom correspondence should be addressed.
Received: 14 July 2016 / Revised: 3 September 2016 / Accepted: 20 September 2016 / Published: 27 September 2016
(This article belongs to the Special Issue Polyphenols for Cancer Treatment or Prevention)
Abstract
To investigate the antitumor effect of anthocyanins extracted from Chinese bayberry fruit (Myrica rubra Sieb. et Zucc.), a nude mouse tumor xenograft model was established. Treatments with C3G (cyanidin-3-glucoside, an anthocyanin) significantly suppressed the growth of SGC-7901 tumor xenografts in a dose-dependent manner. Immunohistochemical staining showed a significant increase in p21 expression, indicating that the cell cycle of tumor xenografts was inhibited. qPCR screening showed that C3G treatment up-regulated the expression of the KLF6 gene, which is an important tumor suppressor gene inactivated in many human cancers. Western blot showed that C3G treatments markedly increased KLF6 and p21 protein levels, inhibited CDK4 and Cyclin D1 expression, but did not notably change the expression of p53. These results indicated that KLF6 up-regulates p21 in a p53-independent manner and significantly reduces tumor proliferation. This study provides important information for the possible mechanism of C3G-induced antitumor activity against gastric adenocarcinoma in vivo. View Full-TextKeywords:
Chinese bayberry; anthocyanin; SGC-7901 cell; tumor xenograft; KLF6 gene
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Wang, Y.; Zhang, X.-N.; Xie, W.-H.; Zheng, Y.-X.; Cao, J.-P.; Cao, P.-R.; Chen, Q.-J.; Li, X.; Sun, C.-D. The Growth of SGC-7901 Tumor Xenografts Was Suppressed by Chinese Bayberry Anthocyanin Extract through Upregulating KLF6 Gene Expression. Nutrients 2016, 8, 599.
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