Next Article in Journal
Heart Rate Variability and Cognitive Function Following a Multi-Vitamin and Mineral Supplementation with Added Guarana (Paullinia cupana)
Previous Article in Journal
A Review of the Impact of Dietary Intakes in Human Pregnancy on Infant Birthweight
Article Menu

Export Article

Open AccessArticle
Nutrients 2015, 7(1), 179-195; doi:10.3390/nu7010179

Piper and Vismia Species from Colombian Amazonia Differentially Affect Cell Proliferation of Hepatocarcinoma Cells

Department of Physiology, Medicine and Dentistry School, University of the Basque Country UPV/EHU, Leioa 48940, Spain
*
Author to whom correspondence should be addressed.
Received: 10 November 2014 / Accepted: 18 December 2014 / Published: 30 December 2014
View Full-Text   |   Download PDF [212 KB, uploaded 30 December 2014]   |  

Abstract

There is an increasing interest to identify plant-derived natural products with antitumor activities. In this work, we have studied the effects of aqueous leaf extracts from Amazonian Vismia and Piper species on human hepatocarcinoma cell toxicity. Results showed that, depending on the cell type, the plants displayed differential effects; thus, Vismia baccifera induced the selective killing of HepG2, while increasing cell growth of PLC-PRF and SK-HEP-1. In contrast, these two last cell lines were sensitive to the toxicity by Piper krukoffii and Piper putumayoense, while the Piperaceae did not affect HepG2 growth. All the extracts induced cytotoxicity to rat hepatoma McA-RH7777, but were innocuous (V. baccifera at concentrations < 75 µg/mL) or even protected cells from basal death (P. putumayoense) in primary cultures of rat hepatocytes. In every case, cytotoxicity was accompanied by an intracellular accumulation of reactive oxygen species (ROS). These results provide evidence for the anticancer activities of the studied plants on specific cell lines and suggest that cell killing could be mediated by ROS, thus involving mechanisms independent of the plants free radical scavenging activities. Results also support the use of these extracts of the Vismia and Piper genera with opposite effects as a model system to study the mechanisms of the antitumoral activity against different types of hepatocarcinoma. View Full-Text
Keywords: antioxidant activity; free radical; polyphenol; hepatoma cell line; cell cycle arrest; flow cytometry; superoxide dismutase; catalase antioxidant activity; free radical; polyphenol; hepatoma cell line; cell cycle arrest; flow cytometry; superoxide dismutase; catalase
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Lizcano, L.J.; Siles, M.; Trepiana, J.; Hernández, M.L.; Navarro, R.; Ruiz-Larrea, M.B.; Ruiz-Sanz, J.I. Piper and Vismia Species from Colombian Amazonia Differentially Affect Cell Proliferation of Hepatocarcinoma Cells. Nutrients 2015, 7, 179-195.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top