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Nutrients 2014, 6(3), 1115-1127; doi:10.3390/nu6031115
Article

Effect of the Novel Polysaccharide PolyGlycopleX® on Short-Chain Fatty Acid Production in a Computer-Controlled in Vitro Model of the Human Large Intestine

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Received: 9 January 2014; in revised form: 11 February 2014 / Accepted: 27 February 2014 / Published: 14 March 2014
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Abstract: Many of the health benefits associated with dietary fiber are attributed to their fermentation by microbiota and production of short chain fatty acids (SCFA). The aim of this study was to investigate the fermentability of the functional fiber PolyGlyopleX® (PGX®) in vitro. A validated dynamic, computer-controlled in vitro system simulating the conditions in the proximal large intestine (TIM-2) was used. Sodium hydroxide (NaOH) consumption in the system was used as an indicator of fermentability and SCFA and branched chain fatty acids (BCFA) production was determined. NaOH consumption was significantly higher for Fructooligosaccharide (FOS) than PGX, which was higher than cellulose (p = 0.002). At 32, 48 and 72 h, acetate and butyrate production were higher for FOS and PGX versus cellulose. Propionate production was higher for PGX than cellulose at 32, 48, 56 and 72 h and higher than FOS at 72 h (p = 0.014). Total BCFA production was lower for FOS compared to cellulose, whereas production with PGX was lower than for cellulose at 72 h. In conclusion, PGX is fermented by the colonic microbiota which appeared to adapt to the substrate over time. The greater propionate production for PGX may explain part of the cholesterol-lowering properties of PGX seen in rodents and humans.
Keywords: dietary fiber; functional fiber; microbial fermentation; polysaccharide; propionate dietary fiber; functional fiber; microbial fermentation; polysaccharide; propionate
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Reimer, R.A.; Maathuis, A.J.H.; Venema, K.; Lyon, M.R.; Gahler, R.J.; Wood, S. Effect of the Novel Polysaccharide PolyGlycopleX® on Short-Chain Fatty Acid Production in a Computer-Controlled in Vitro Model of the Human Large Intestine. Nutrients 2014, 6, 1115-1127.

AMA Style

Reimer RA, Maathuis AJH, Venema K, Lyon MR, Gahler RJ, Wood S. Effect of the Novel Polysaccharide PolyGlycopleX® on Short-Chain Fatty Acid Production in a Computer-Controlled in Vitro Model of the Human Large Intestine. Nutrients. 2014; 6(3):1115-1127.

Chicago/Turabian Style

Reimer, Raylene A.; Maathuis, Annet J.H.; Venema, Koen; Lyon, Michael R.; Gahler, Roland J.; Wood, Simon. 2014. "Effect of the Novel Polysaccharide PolyGlycopleX® on Short-Chain Fatty Acid Production in a Computer-Controlled in Vitro Model of the Human Large Intestine." Nutrients 6, no. 3: 1115-1127.


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