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Nutrients 2014, 6(12), 5667-5678; doi:10.3390/nu6125667

Nrf2-Mediated HO-1 Induction Contributes to Antioxidant Capacity of a Schisandrae Fructus Ethanol Extract in C2C12 Myoblasts

Blue-Bio Industry Regional Innovation Center and Anti-Aging Research Center, Dongeui University, Busan 614-714, Korea
Department of Biochemistry, College of Korean Medicine, Dongeui University, Busan 614-052, Korea
Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Korea
Department of Biochemistry, College of Medicine, Busan National University, Yangsan 626-870, Korea
Department of Life Science and Biotechnology, College of Natural Sciences, Dongeui University, Busan 614-714, Korea
Department of Food and Nutrition, College of Human Ecology, Dongeui University, Busan 614-714, Korea
Author to whom correspondence should be addressed.
Received: 8 October 2014 / Revised: 16 November 2014 / Accepted: 24 November 2014 / Published: 8 December 2014
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This study was designed to confirm the protective effect of Schisandrae Fructus, which are the dried fruits of Schisandra chinensis (Turcz.) Baill, against oxidative stress-induced cellular damage and to elucidate the underlying mechanisms in C2C12 myoblasts. Preincubating C2C12 cells with a Schisandrae Fructus ethanol extract (SFEE) significantly attenuated hydrogen peroxide (H2O2)-induced inhibition of growth and induced scavenging activity against intracellular reactive oxygen species (ROS) induced by H2O2. SFEE also inhibited comet tail formation and phospho-histone γH2A.X expression, suggesting that it prevents H2O2-induced cellular DNA damage. Furthermore, treating C2C12 cells with SFEE significantly induced heme oxygenase-1 (HO-1) and phosphorylation of nuclear factor-erythroid 2 related factor 2 (Nrf2). However, zinc protoporphyrin IX, a potent inhibitor of HO-1 activity, significantly reversed the protective effects of SFEE against H2O2-induced growth inhibition and ROS generation in C2C12 cells. Additional experiments revealed that the potential of the SFEE to induce HO-1 expression and protect against H2O2-mediated cellular damage was abrogated by transient transfection with Nrf2-specific small interfering RNA, suggesting that the SFEE protected C2C12 cells against oxidative stress-induced injury through the Nrf2/HO-1 pathway. View Full-Text
Keywords: Schisandrae Fructus; oxidative stress; ROS; Nrf2/HO-1 Schisandrae Fructus; oxidative stress; ROS; Nrf2/HO-1

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kang, J.S.; Han, M.H.; Kim, G.-Y.; Kim, C.M.; Kim, B.W.; Hwang, H.J.; Choi, Y.H. Nrf2-Mediated HO-1 Induction Contributes to Antioxidant Capacity of a Schisandrae Fructus Ethanol Extract in C2C12 Myoblasts. Nutrients 2014, 6, 5667-5678.

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