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Nutrients 2014, 6(12), 5652-5666; doi:10.3390/nu6125652

A Novel Hemp Seed Meal Protein Hydrolysate Reduces Oxidative Stress Factors in Spontaneously Hypertensive Rats

1
Department of Human Nutritional Sciences and the Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
2
School of Agriculture and Wine Sciences, Charles Sturt University, Bag 588, Wagga Wagga, NSW 2678, Australia
3
College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210046, China
4
Department of Physiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
5
Canadian Centre for Agri-Food Research in Health and Medicine, 351 Tache Avenue, Winnipeg, MB R2H 2A6, Canada
*
Author to whom correspondence should be addressed.
Received: 7 August 2014 / Revised: 2 November 2014 / Accepted: 24 November 2014 / Published: 8 December 2014
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Abstract

This report shows the antioxidant effects of a hemp seed meal protein hydrolysate (HMH) in spontaneously hypertensive rats (SHR). Defatted hemp seed meal was hydrolyzed consecutively with pepsin and pancreatin to yield HMH, which was incorporated into rat feed as a source of antioxidant peptides. Young (8-week old) SHRs were divided into three groups (8 rats/group) and fed diets that contained 0.0%, 0.5% or 1.0% (w/w) HMH for eight weeks; half of the rats were sacrificed for blood collection. After a 4-week washout period, the remaining 20-week old SHRs were fed for an additional four weeks and sacrificed for blood collection. Plasma total antioxidant capacity (TAC) and superoxide dismutase (SOD), catalase (CAT) and total peroxides (TPx) levels were determined. Results showed that plasma TAC, CAT and SOD levels decreased in the older 20-week old SHRs when compared to the young SHRs. The presence of HMH in the diets led to significant (p < 0.05) increases in plasma SOD and CAT levels in both young and adult SHR groups; these increases were accompanied by decreases in TPx levels. The results suggest that HMH contained antioxidant peptides that reduced the rate of lipid peroxidation in SHRs with enhanced antioxidant enzyme levels and total antioxidant capacity. View Full-Text
Keywords: hemp seed; antioxidant peptides; protein hydrolysate; spontaneously hypertensive rats; total antioxidant capacity; pepsin; pancreatin; defatted meal hemp seed; antioxidant peptides; protein hydrolysate; spontaneously hypertensive rats; total antioxidant capacity; pepsin; pancreatin; defatted meal
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Girgih, A.T.; Alashi, A.M.; He, R.; Malomo, S.A.; Raj, P.; Netticadan, T.; Aluko, R.E. A Novel Hemp Seed Meal Protein Hydrolysate Reduces Oxidative Stress Factors in Spontaneously Hypertensive Rats. Nutrients 2014, 6, 5652-5666.

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