Nutrients 2013, 5(12), 4894-4907; doi:10.3390/nu5124894
Article

Phenolic Composition, Antioxidant Activity and Anti-Adipogenic Effect of Hot Water Extract from Safflower (Carthamus tinctorius L.) Seed

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Received: 19 June 2013; in revised form: 12 November 2013 / Accepted: 20 November 2013 / Published: 28 November 2013
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: This study was to evaluate the phenolic content and composition of Carthamus tinctorius L. seed extract (CSE) and to further assess its antioxidant and anti-adipogenic activities using various radical scavenging systems and 3T3-L1 cells. Our results show that the total phenolic and flavonoid contents of CSE were 126.0 ± 2.4 mg GAE/g and 62.2 ± 1.9 mg QE/g, respectively. The major phenolic compounds in CSE was (−)-epigallocatechin (109.62 mg/g), with a 4-hydroxy benzhydrazide derivative and gallocatechin present at 18.28 mg/g and 17.02 mg/g, respectively. CSE exhibited remarkable radical scavenging activities, FRAP (ferric reducing antioxidant power) and reducing power in a dose-dependent manner. Moreover, the oxygen radical absorbance capacity (ORAC) value of CSE (0.1 mg/mL) was 62.9 ± 4.7 μM TE (trolox equivalent)/g. During adipogenesis, CSE significantly inhibited fat accumulation in 3T3-L1 cells compared with control cells. Overall, these results indicate that CSE might be a valuable source of bioactive compounds that impart functional food and natural antioxidant properties.
Keywords: antioxidant activity; phenolic compound; Carthamus tinctorius L. seed; 3T3-L1 adipocyte; hot-water extract
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MDPI and ACS Style

Yu, S.-Y.; Lee, Y.-J.; Kim, J.-D.; Kang, S.-N.; Lee, S.-K.; Jang, J.-Y.; Lee, H.-K.; Lim, J.-H.; Lee, O.-H. Phenolic Composition, Antioxidant Activity and Anti-Adipogenic Effect of Hot Water Extract from Safflower (Carthamus tinctorius L.) Seed. Nutrients 2013, 5, 4894-4907.

AMA Style

Yu S-Y, Lee Y-J, Kim J-D, Kang S-N, Lee S-K, Jang J-Y, Lee H-K, Lim J-H, Lee O-H. Phenolic Composition, Antioxidant Activity and Anti-Adipogenic Effect of Hot Water Extract from Safflower (Carthamus tinctorius L.) Seed. Nutrients. 2013; 5(12):4894-4907.

Chicago/Turabian Style

Yu, Seok-Yeong; Lee, Young-Jun; Kim, Jong-Dai; Kang, Suk-Nam; Lee, Seong-Kap; Jang, Jung-Young; Lee, Hyo-Ku; Lim, Jeong-Ho; Lee, Ok-Hwan. 2013. "Phenolic Composition, Antioxidant Activity and Anti-Adipogenic Effect of Hot Water Extract from Safflower (Carthamus tinctorius L.) Seed." Nutrients 5, no. 12: 4894-4907.


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