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Investigation of Effect of Nutritional Drink on Chemotherapy-Induced Mucosal Injury and Tumor Growth in an Established Animal Model
Mucositis Research Group, Centre for Personalised Cancer Medicine (CPCM), Centre for Clinical Research Excellence (CCRE) in Oral Health, Faculty of Health Sciences, University of Adelaide, Frome Road, Adelaide, SA 5005, Australia
School of Pharmacy & Medical Sciences, University of South Australia, City East Campus, Frome Road, Adelaide, SA 5005, Australia
Nestlé Nutrition R&D Centers, 12500 Whitewater Drive, Minnetonka, MN 55343, USA
Nestlé Research Centre, Nestec Ltd., Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland
RAH Cancer Centre, Royal Adelaide Hospital, Adelaide SA 5000, Australia
* Author to whom correspondence should be addressed.
Received: 8 August 2013; in revised form: 17 September 2013 / Accepted: 22 September 2013 / Published: 30 September 2013
Abstract: Chemotherapy-induced mucositis represents a significant burden to quality of life and healthcare costs, and may be improved through enhanced nutritional status. We first determined the safety of two nutritional drinks (plus placebo), and then potential gut protection in tumor-bearing rats in a model of methotrexate-induced mucositis. In study 1, animals were fed one of two test diets (or placebo or control chow pellets) for a total of 60 days and were monitored daily. All diets were found to be safe to administer. In study 2, after seven days of receiving diets, a Dark Agouti Mammary Adenocarcinoma (DAMA) was transplanted subcutaneously. Ten days after starting diets, animals had 2 mg/kg intramuscular methotrexate administered on two consecutive days; after this time, all animals were given soaked chow. Animals were monitored daily for changes in bodyweight, tumor burden and general health. Animals were killed 10, 12 and 16 days after initially starting diets, and tissues were collected at necropsy. In study 1, animals receiving diets had gained 0.8% and 10.8% of their starting bodyweight after 60 days, placebo animals 4.4%, and animals fed on standard chow had gained 15.1%. In study 2, there was no significant influence of test diet on bodyweight, organ weight, tumor burden or biochemical parameters. Only animals treated with MTX exhibited diarrhea, although animals receiving Diet A and Diet C showed a non-significant increase in incidence of diarrhea. Administration of these nutritional drinks did not improve symptoms of mucositis.
Keywords: mucositis; nutritional drinks; animal models
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Bateman, E.; Bowen, J.; Stringer, A.; Mayo, B.; Plews, E.; Wignall, A.; Greenberg, N.; Schiffrin, E.; Keefe, D. Investigation of Effect of Nutritional Drink on Chemotherapy-Induced Mucosal Injury and Tumor Growth in an Established Animal Model. Nutrients 2013, 5, 3948-3963.
Bateman E, Bowen J, Stringer A, Mayo B, Plews E, Wignall A, Greenberg N, Schiffrin E, Keefe D. Investigation of Effect of Nutritional Drink on Chemotherapy-Induced Mucosal Injury and Tumor Growth in an Established Animal Model. Nutrients. 2013; 5(10):3948-3963.
Bateman, Emma; Bowen, Joanne; Stringer, Andrea; Mayo, Bronwen; Plews, Erin; Wignall, Anthony; Greenberg, Norman; Schiffrin, Eduardo; Keefe, Dorothy. 2013. "Investigation of Effect of Nutritional Drink on Chemotherapy-Induced Mucosal Injury and Tumor Growth in an Established Animal Model." Nutrients 5, no. 10: 3948-3963.