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Nutrients 2010, 2(1), 1-15; doi:10.3390/nu2010001
Article

NMR-Metabolic Methodology in the Study of GM Foods

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Received: 8 December 2009; Accepted: 8 January 2010 / Published: 13 January 2010
(This article belongs to the Special Issue Foodomics 2009)
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Abstract: The 1H-NMR methodology used in the study of genetically modified (GM) foods is discussed. Transgenic lettuce (Lactuca sativa cv \"Luxor\") over-expressing the Arabidopsis KNAT1 gene is presented as a case study. Twenty-two water-soluble metabolites (amino acids, organic acids, sugars) present in leaves of conventional and GM lettuce were monitored by NMR and quantified at two developmental stages. The NMR spectra did not reveal any difference in metabolite composition between the GM lettuce and the wild type counterpart. Statistical analyses of metabolite variables highlighted metabolism variation as a function of leaf development as well as the transgene. A main effect of the transgene was in altering sugar metabolism.
Keywords: NMR metabolic profiling; genetically modified food; transgenic lettuce NMR metabolic profiling; genetically modified food; transgenic lettuce
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Sobolev, A.P.; Capitani, D.; Giannino, D.; Nicolodi, C.; Testone, G.; Santoro, F.; Frugis, G.; Iannelli, M.A.; Mattoo, A.K.; Brosio, E.; Gianferri, R.; D’Amico, I.; Mannina, L. NMR-Metabolic Methodology in the Study of GM Foods. Nutrients 2010, 2, 1-15.

AMA Style

Sobolev AP, Capitani D, Giannino D, Nicolodi C, Testone G, Santoro F, Frugis G, Iannelli MA, Mattoo AK, Brosio E, Gianferri R, D’Amico I, Mannina L. NMR-Metabolic Methodology in the Study of GM Foods. Nutrients. 2010; 2(1):1-15.

Chicago/Turabian Style

Sobolev, Anatoly P.; Capitani, Donatella; Giannino, Donato; Nicolodi, Chiara; Testone, Giulio; Santoro, Flavio; Frugis, Giovanna; Iannelli, Maria A.; Mattoo, Autar K.; Brosio, Elvino; Gianferri, Raffaella; D’Amico, Irene; Mannina, Luisa. 2010. "NMR-Metabolic Methodology in the Study of GM Foods." Nutrients 2, no. 1: 1-15.



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