Nutrients 2010, 2(1), 1-15; doi:10.3390/nu2010001
Article

NMR-Metabolic Methodology in the Study of GM Foods

Anatoly P. Sobolev 1,* email, Donatella Capitani 1 email, Donato Giannino 2 email, Chiara Nicolodi 2 email, Giulio Testone 2 email, Flavio Santoro 1 email, Giovanna Frugis 2 email, Maria A. Iannelli 2 email, Autar K. Mattoo 3 email, Elvino Brosio 4 email, Raffaella Gianferri 4 email, Irene D’Amico 5 email and Luisa Mannina 1,6,* email
1 Institute of Chemical Methodologies, Magnetic Resonance Laboratory “Annalaura Segre”, CNR, Via Salaria km 29.300, 00015 Monterotondo, Rome, Italy 2 Institute of Biology and Agricultural Biotechnology, CNR, Via Salaria km 29.300, 00015 Monterotondo, Rome, Italy 3 Henry A. Wallace Beltsville Agricultural Research Center, United States Department of Agriculture, Agricultural Research Service, Sustainable Agricultural Systems Laboratory, Beltsville, Maryland 20705–2350, USA 4 Chemistry Department, University of Rome “Sapienza”, p.le Aldo Moro, 5 00185, Rome, Italy 5 STAAM Department, University of Molise, Via De Sanctis, 86100 Campobasso, Italy 6 Faculty of Pharmacy, University of Rome “Sapienza”, p.le Aldo Moro, 5 00185, Rome, Italy
* Authors to whom correspondence should be addressed.
Received: 8 December 2009 / Accepted: 8 January 2010 / Published: 13 January 2010
(This article belongs to the Special Issue Foodomics 2009)
PDF Full-text Download PDF Full-Text [291 KB, uploaded 14 January 2010 08:59 CET]
Abstract: The 1H-NMR methodology used in the study of genetically modified (GM) foods is discussed. Transgenic lettuce (Lactuca sativa cv \"Luxor\") over-expressing the Arabidopsis KNAT1 gene is presented as a case study. Twenty-two water-soluble metabolites (amino acids, organic acids, sugars) present in leaves of conventional and GM lettuce were monitored by NMR and quantified at two developmental stages. The NMR spectra did not reveal any difference in metabolite composition between the GM lettuce and the wild type counterpart. Statistical analyses of metabolite variables highlighted metabolism variation as a function of leaf development as well as the transgene. A main effect of the transgene was in altering sugar metabolism.
Keywords: NMR metabolic profiling; genetically modified food; transgenic lettuce

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Sobolev, A.P.; Capitani, D.; Giannino, D.; Nicolodi, C.; Testone, G.; Santoro, F.; Frugis, G.; Iannelli, M.A.; Mattoo, A.K.; Brosio, E.; Gianferri, R.; D’Amico, I.; Mannina, L. NMR-Metabolic Methodology in the Study of GM Foods. Nutrients 2010, 2, 1-15.

AMA Style

Sobolev AP, Capitani D, Giannino D, Nicolodi C, Testone G, Santoro F, Frugis G, Iannelli MA, Mattoo AK, Brosio E, Gianferri R, D’Amico I, Mannina L. NMR-Metabolic Methodology in the Study of GM Foods. Nutrients. 2010; 2(1):1-15.

Chicago/Turabian Style

Sobolev, Anatoly P.; Capitani, Donatella; Giannino, Donato; Nicolodi, Chiara; Testone, Giulio; Santoro, Flavio; Frugis, Giovanna; Iannelli, Maria A.; Mattoo, Autar K.; Brosio, Elvino; Gianferri, Raffaella; D’Amico, Irene; Mannina, Luisa. 2010. "NMR-Metabolic Methodology in the Study of GM Foods." Nutrients 2, no. 1: 1-15.

Nutrients EISSN 2072-6643 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert