Anti-Inflammatory and Gut Microbiota Modulating Effects of Probiotic Lactobacillus paracasei MSMC39-1 on Dextran Sulfate Sodium-Induced Colitis in Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Probiotic Culture Supernatant and Viable Cells Preparation
2.2. Heat-Killed Cells Preparation
2.3. Sonicated Cells Preparation
2.4. Caco-2 Cells
2.5. The effect of Probiotic Strains in the TNF-α Production in Caco-2 Cells
2.6. Animal Experiment
2.7. Induction of Colitis
2.8. Experimental Designs
2.9. Detection of Aspartate Aminotransferase and Alanine Aminotransferase
2.10. Detection of TNF-α in the Colon and Hepatic Tissue
2.11. Histology Evaluation of Colitis Rats
2.12. Microbiota Detection using 16S rDNA Next-Generation Sequencing (NGS)
2.13. Statistical Analysis
3. Results
3.1. The effect of Probiotic Strains on TNF-α Production in Caco-2 Cells
3.2. Effect of Probiotic L. paracasei Strain MSMC39-1 on Body Weight and Stool Consistency in DSS-Induced Colitis Rats
3.3. Effect of Probiotic L. paracasei Strain MSMC39-1 on Liver Functional Enzyme Activity in DSS-Induced Colitis Rats
3.4. Effect of Probiotic L. paracasei Strain MSMC39-1 on TNF-α Production in Colon and Liver Tissues of DSS-Induced Colitis Rats
3.5. Effect of Probiotic L. paracasei Strain MSMC39-1 on Colon and Liver Tissue Histology in DSS-Induced Colitis Rats
3.6. Effect of L. paracasei Strain MSMC39-1 on Microbiota Modulation in DSS-Induced Colitis Rats
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Score | Characteristics |
---|---|
0 | no ulcer and inflammation |
1 | no ulcer and local hyperemia |
2 | ulcer without hyperemia |
3 | ulcer and one site only of inflammation |
4 | two or more sites of ulcer and inflammation |
5 | ulcer extending more than 2 cm |
Group | ||||
---|---|---|---|---|
Parameters | Negative Control | Probiotic Control | Colitis Control | Probiotic Test |
Body weight increase (g) | 125.00 ± 9.98 | 122.50 ± 23.91 | 93.41 ± 6.80 | 107.40 ± 11.03 |
Stool consistency | Formed | Formed | Loose, bloody | Formed |
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Ladda, B.; Jantararussamee, C.; Pradidarcheep, W.; Kasorn, A.; Matsathit, U.; Taweechotipatr, M. Anti-Inflammatory and Gut Microbiota Modulating Effects of Probiotic Lactobacillus paracasei MSMC39-1 on Dextran Sulfate Sodium-Induced Colitis in Rats. Nutrients 2023, 15, 1388. https://doi.org/10.3390/nu15061388
Ladda B, Jantararussamee C, Pradidarcheep W, Kasorn A, Matsathit U, Taweechotipatr M. Anti-Inflammatory and Gut Microbiota Modulating Effects of Probiotic Lactobacillus paracasei MSMC39-1 on Dextran Sulfate Sodium-Induced Colitis in Rats. Nutrients. 2023; 15(6):1388. https://doi.org/10.3390/nu15061388
Chicago/Turabian StyleLadda, Boonyarut, Chittapon Jantararussamee, Wisuit Pradidarcheep, Anongnard Kasorn, Udomlak Matsathit, and Malai Taweechotipatr. 2023. "Anti-Inflammatory and Gut Microbiota Modulating Effects of Probiotic Lactobacillus paracasei MSMC39-1 on Dextran Sulfate Sodium-Induced Colitis in Rats" Nutrients 15, no. 6: 1388. https://doi.org/10.3390/nu15061388