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Pharmaceutics 2016, 8(3), 29; doi:10.3390/pharmaceutics8030029

Rapid Quantification and Validation of Lipid Concentrations within Liposomes

1
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow G4 0RE, UK
2
Aston Pharmacy School, School of Life and Health Sciences, Aston University, Birmingham B4 7ET, UK
3
School of Pharmacy and Pharmaceutical Sciences, Ulster University, Coleraine BT52 1SA, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Natasa Skalko-Basnet
Received: 14 July 2016 / Revised: 18 August 2016 / Accepted: 2 September 2016 / Published: 13 September 2016
(This article belongs to the Special Issue Liposome Technologies 2015)
View Full-Text   |   Download PDF [1963 KB, uploaded 13 September 2016]   |  

Abstract

Quantification of the lipid content in liposomal adjuvants for subunit vaccine formulation is of extreme importance, since this concentration impacts both efficacy and stability. In this paper, we outline a high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method that allows for the rapid and simultaneous quantification of lipid concentrations within liposomal systems prepared by three liposomal manufacturing techniques (lipid film hydration, high shear mixing, and microfluidics). The ELSD system was used to quantify four lipids: 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), cholesterol, dimethyldioctadecylammonium (DDA) bromide, and ᴅ-(+)-trehalose 6,6′-dibehenate (TDB). The developed method offers rapidity, high sensitivity, direct linearity, and a good consistency on the responses (R2 > 0.993 for the four lipids tested). The corresponding limit of detection (LOD) and limit of quantification (LOQ) were 0.11 and 0.36 mg/mL (DMPC), 0.02 and 0.80 mg/mL (cholesterol), 0.06 and 0.20 mg/mL (DDA), and 0.05 and 0.16 mg/mL (TDB), respectively. HPLC-ELSD was shown to be a rapid and effective method for the quantification of lipids within liposome formulations without the need for lipid extraction processes. View Full-Text
Keywords: lipids; liposomes; cholesterol; quantification; HPLC; ELSD lipids; liposomes; cholesterol; quantification; HPLC; ELSD
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Roces, C.B.; Kastner, E.; Stone, P.; Lowry, D.; Perrie, Y. Rapid Quantification and Validation of Lipid Concentrations within Liposomes. Pharmaceutics 2016, 8, 29.

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